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PMID: 8144468 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Detailed structural characterization of succinoglycan, the major exopolysaccharide of Rhizobium meliloti Rm1021.

Journal of bacteriology ·Vol. 176 ·No. 7 ·1994-04-00 ·Pages 1997-2002

Reinhold BB, Chan SY, Reuber TL, Marra A, Walker GC, Reinhold VN

Abstract

The detailed structure of the symbiotically important exopolysaccharide succinoglycan from Rhizobium meliloti Rm1021 was determined by mass spectrometry with electrospray ionization and collision-induced dissociation of the octameric oligosaccharide repeating unit. Previously undetermined locations of the succinyl and acetyl modifications were determined, in respect to both residue locations within the octamer and the carbon positions within the pyranose ring. Glycosidic linkages determined previously by methylation analysis were also verified.

MeSH Terms

Carbohydrate Sequence Gas Chromatography-Mass Spectrometry Molecular Sequence Data Oligosaccharides/chemistry Polysaccharides, Bacterial/chemistry Sinorhizobium meliloti/chemistry

Chemicals

Oligosaccharides Polysaccharides, Bacterial succinoglycan

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Reinhold B B

Department of Nutrition, Harvard School of Public Health, Boston, Massachusetts 02115.

Chan S Y

Reuber T L

Marra A

Walker G C

Reinhold V N

References (10)

10 references, click to expand

Studies on an extracellular polysaccharide from Rhizobium meliloti.
Acta Chem Scand. 1971;25(4):1281-6 PMID: 5112437
Demonstration of an octasaccharide repeating unit in the extracellular polysaccharide of Rhizobium meliloti by sequential degradation.
J Am Chem Soc. 1977 May 25;99(11):3812-5 PMID: 858877
Methylation analysis of complex carbohydrates in small amounts: capillary gas chromatography-mass fragmentography of methylalditol acetates obtained from N-glycosidically linked glycoprotein oligosaccharides.
Anal Biochem. 1983 Aug;133(1):197-207 PMID: 6638480
Exopolysaccharide-deficient mutants of Rhizobium meliloti that form ineffective nodules.
Proc Natl Acad Sci U S A. 1985 Sep;82(18):6231-5 PMID: 3862129
The acetyl substituent of succinoglycan is not necessary for alfalfa nodule invasion by Rhizobium meliloti Rm1021.
J Bacteriol. 1993 Jun;175(11):3653-5 PMID: 8501069
A novel exopolysaccharide can function in place of the calcofluor-binding exopolysaccharide in nodulation of alfalfa by Rhizobium meliloti.
Cell. 1989 Feb 24;56(4):661-72 PMID: 2537152
Electrospray ionization for mass spectrometry of large biomolecules.
Science. 1989 Oct 6;246(4926):64-71 PMID: 2675315
Structural studies of a novel exopolysaccharide produced by a mutant of Rhizobium meliloti strain Rm1021.
Carbohydr Res. 1990 May 1;198(2):305-12 PMID: 2379191
Exopolysaccharides in plant-bacterial interactions.
Annu Rev Microbiol. 1992;46:307-46 PMID: 1444258
Rhizobium meliloti mutants that fail to succinylate their calcofluor-binding exopolysaccharide are defective in nodule invasion.
Cell. 1987 Nov 20;51(4):579-87 PMID: 2824062

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.176.7.1997-2002.1994

Published

1994-04-00

Pages

1997-2002

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC205305

Subset

Grants

NIGMS NIH HHS · GM-31030 · United States

NIGMS NIH HHS · R01 GM-45781 · United States

External Links

PubMed source DOI: 10.1128/jb.176.7.1997-2002.1994 PMC Full Text View journal details

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Detailed structural characterization of succinoglycan, the major exopolysaccharide of Rhizobium meliloti Rm1021.

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