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PMID: 8114732 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of two steps during Xenopus ribosomal gene transcription that are sensitive to protein phosphorylation.

Molecular and cellular biology ·Vol. 14 ·No. 3 ·1994-03-00 ·Pages 2011-20

Labhart P

Abstract

Protein kinase(s) and protein phosphatase(s) present in a Xenopus S-100 transcription extract strongly influence promoter-dependent transcription by RNA polymerase I. The protein kinase inhibitor 6-dimethyl-aminopurine causes transcription to increase, while the protein phosphatase inhibitor okadaic acid causes transcription to decrease. Repression is also observed with inhibitor 2, and the addition of extra protein phosphatase 1 stimulates transcription, indicating that the endogenous phosphatase is a type 1 enzyme. Partial fractionation of the system, single-round transcription reactions, and kinetic experiments show that two different steps during ribosomal gene transcription are sensitive to protein phosphorylation: okadaic acid affects a step before or during transcription initiation, while 6-dimethylaminopurine stimulates a process "late" in the reaction, possibly reinitiation. The present results are a clear demonstration that transcription by RNA polymerase I can be regulated by protein phosphorylation.

MeSH Terms
Adenine/analogs & derivatives,pharmacology Animals Cell-Free System DNA, Ribosomal/genetics Ethers, Cyclic/pharmacology Gene Expression Regulation In Vitro Techniques Okadaic Acid Phosphoprotein Phosphatases/antagonists & inhibitors,metabolism Phosphoproteins/physiology Protein Kinase Inhibitors Protein Kinases/metabolism Protein Phosphatase 1 RNA Polymerase I/metabolism RNA, Ribosomal/biosynthesis Transcription, Genetic Xenopus laevis/genetics
Chemicals
DNA, Ribosomal Ethers, Cyclic Phosphoproteins Protein Kinase Inhibitors RNA, Ribosomal Okadaic Acid N(6),N(6)-dimethyladenine Protein Kinases RNA Polymerase I Phosphoprotein Phosphatases Protein Phosphatase 1 Adenine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Labhart P
Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037.
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43 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.3.2011-2020.1994
Published
1994-03-00
Pages
2011-20
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358561
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.14.3.2011-2020.1994 PMC Full Text View journal details
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