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PMID: 8113199 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Effect of iron availability on expression of the Bradyrhizobium japonicum hemA gene.

Journal of bacteriology ·Vol. 176 ·No. 5 ·1994-03-00 ·Pages 1535-8

Page KM, Connolly EL, Guerinot ML

Abstract

Bradyrhizobium japonicum produces delta-aminolevulinic acid, the universal precursor of tetrapyrroles, in a reaction catalyzed by the product of the hemA gene. Expression of the B. japonicum hemA gene is affected by iron availability. Activity of a hemA-lacZ fusion is increased approximately threefold by iron, and RNA analysis indicates that iron regulation is at the level of mRNA accumulation. To our knowledge, this is the first example of an iron-regulated heme biosynthetic gene in prokaryotes.

Related Genes
fur hemA iucA lacZ
MeSH Terms
Aldehyde Oxidoreductases/biosynthesis,genetics,metabolism Amino Acid Sequence Aminolevulinic Acid/metabolism Bacterial Proteins/biosynthesis,genetics,metabolism Base Sequence Consensus Sequence Escherichia coli/genetics,metabolism Gene Expression Regulation, Bacterial/drug effects Genes, Bacterial Iron/metabolism,pharmacology Molecular Sequence Data RNA, Messenger/biosynthesis,metabolism Recombinant Fusion Proteins/metabolism Restriction Mapping Rhizobiaceae/drug effects,genetics,metabolism Sequence Homology, Nucleic Acid Transcription, Genetic beta-Galactosidase/metabolism
Chemicals
Bacterial Proteins RNA, Messenger Recombinant Fusion Proteins Aminolevulinic Acid Iron Aldehyde Oxidoreductases glutamyl tRNA reductase beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Page K M
Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755.
Connolly E L
Guerinot M L
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.5.1535-1538.1994
Published
1994-03-00
Pages
1535-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205226
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.176.5.1535-1538.1994 PMC Full Text View journal details
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