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PMID: 8108417 Published · ppublish English Comparative Study Journal Article

Pseudomonas aeruginosa possesses homologues of mammalian phenylalanine hydroxylase and 4 alpha-carbinolamine dehydratase/DCoH as part of a three-component gene cluster.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 4 ·1994-02-15 ·Pages 1366-70

Zhao G, Xia T, Song J, Jensen RA

Abstract

Pseudomonas aeruginosa possesses a multigene operon that includes phenylalanine hydroxylase (PhhA; phenylalanine 4-monooxygenase, EC 1.14.16.1). phhA encodes PhhA (M(r) = 30,288), phhB (M(r) = 13,333) encodes a homologue of mammalian 4 alpha-carbinolamine dehydratase/homeodomain protein transregulator, and phhC encodes an aromatic aminotransferase (M(r) = 43,237). The reading frames specifying phhB and phhC overlap by 2 bases. The P. aeruginosa PhhA appears to contain iron and is pterin dependent. Unlike the multimeric mammalian hydroxylase, the native P. aeruginosa enzyme is a monomer. The P. aeruginosa PhhA is homologous with mammalian PhhA, tryptophan hydroxylase, and tyrosine hydroxylase. Expression of PhhA from its native promoter required phhB. This may suggest a positive regulatory role for phhB, consistent with the dual catalytic and regulatory roles of the corresponding mammalian homologue.

Related Genes
phhA phhB phhC
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Genes, Bacterial/genetics Hydro-Lyases/genetics Molecular Sequence Data Multigene Family/genetics Operon/genetics Phenylalanine Hydroxylase/genetics Pseudomonas aeruginosa/genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Transaminases/genetics
Chemicals
Phenylalanine Hydroxylase Transaminases Hydro-Lyases pterin-4a-carbinolamine dehydratase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Zhao G
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.
Xia T
Song J
Jensen R A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.4.1366
Published
1994-02-15
Pages
1366-70
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43159
Subset
IM
Databases
GENBANK
M88627
External Links
PubMed source DOI: 10.1073/pnas.91.4.1366 PMC Full Text View journal details
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