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PMID: 8107222 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Hormonal regulation of the gene for the type C ecotropic retrovirus receptor in rat liver cells.

Journal of virology ·Vol. 68 ·No. 3 ·1994-03-00 ·Pages 1615-23

Wu JY, Robinson D, Kung HJ, Hatzoglou M

Abstract

The infectibility of the regenerating rat liver by ecotropic retroviruses was studied relative to the expression of the gene coding for the ecotropic retrovirus receptor (Ecor) that functions as a cationic amino acid transporter. It is known that the gene for the receptor is expressed in primary hepatocytes and hepatoma cells but is absent in adult liver cells. Isolation of a 2.85-kb cDNA for the rat Ecor suggested that the rat viral receptor is 97% homologous to the mouse viral receptor and that it contains the envelope-binding domain that determines the host range of ecotropic murine retroviruses. This explains the efficient infection of rat cells by ecotropic retroviruses. Since cell division is required for liver cells to be infected, we determined the susceptibility of the regenerating rat liver to infection at different time points after partial hepatectomy (0 to 24 h) in relation to the presence of receptor mRNA. Infection of the liver occurred only when the liver was exposed to virus 4 h after partial hepatectomy. This time course of infection paralleled expression of the gene for the Ecor, which was rapidly induced between 2 and 6 h during liver regeneration. However, expression of the dormant receptor gene in quiescent liver cells can be induced by insulin, dexamethasone, and arginine, indicating that cell division is not required for expression of the receptor gene in liver cells. A diet high in carbohydrate (low in protein) significantly increased the concentration of receptor mRNA in liver cells, indicating that hormones play a role in the regulation of expression of this gene in vivo. We conclude that the gene for the viral receptor is expressed in the regenerating and quiescent liver when the urea cycle enzymes are down regulated. The infection of the regenerating rat liver by ecotropic retroviruses at the time point of expression of the receptor gene supports the requirement of expression of this transporter for infection.

MeSH Terms
Amino Acid Sequence Animals Arginine/pharmacology Base Sequence Carrier Proteins/biosynthesis,genetics Cloning, Molecular Dexamethasone/pharmacology Gene Expression Regulation/drug effects Hormones/pharmacology Insulin/pharmacology Leukemia Virus, Murine/growth & development Leukemia, Experimental/metabolism Liver/cytology,metabolism Liver Regeneration Membrane Glycoproteins Membrane Proteins/biosynthesis,genetics Mice Molecular Sequence Data Rats Rats, Sprague-Dawley Receptors, Virus/biosynthesis,genetics Retroviridae Infections/metabolism Sequence Analysis Sequence Homology, Amino Acid Tumor Cells, Cultured Tumor Virus Infections/metabolism beta-Galactosidase/genetics
Chemicals
Carrier Proteins Hormones Insulin Membrane Glycoproteins Membrane Proteins Receptors, Virus ecotropic murine leukemia virus receptor Dexamethasone Arginine beta-Galactosidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wu J Y
Department of Nutrition, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
Robinson D
Kung H J
Hatzoglou M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.3.1615-1623.1994
Published
1994-03-00
Pages
1615-23
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC236619
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.68.3.1615-1623.1994 PMC Full Text View journal details
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