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题目:: Inhibition of B cell expression of DNA polymerase alpha by CD4+ suppressor T cells.
作者:: Hirohata(S)
状态:: 发布时间1993-09-08 , 更新时间 2014-11-20
期刊:: Cell Immunol
摘要:: Human CD4+ T cells stimulated with immobilized monoclonal antibodies to the CD3 molecular complex (64.1) have been shown to exert suppressive effects on B cell differentiation by inhibiting the G1-S transition of the cell cycle of B cells. The present study investigates the influences of the anti-CD3-induced CD4+ suppressor T cells on the expression of DNA polymerase alpha in B cells. The expression of DNA polymerase alpha in B cells cocultured with immobilized anti-CD3-stimulated CD4+ T cells without mitomycin C treatment (control T4 cells) was significantly decreased after 72 hr of the cultures compared with that in B cells cocultured with immobilized anti-CD3-stimulated CD4+ T cells that had been treated with mitomycin C to abrogate the suppressive effects. The inhibition of the expression of DNA polymerase alpha in B cells was reversible upon removal of anti-CD3-activated control T4 cells from the culture. The B cell expression of proliferating cell nuclear antigen, which is closely related to the auxiliary protein for DNA polymerase delta, was not inhibited by anti-CD3-activated control T4 cells at any time of the cultures. Thus, the results demonstrate that the suppression of human B cell maturation by the anti-CD3-induced CD4+ suppressor T cells is closely related with the inhibition of the expression of DNA polymerase alpha in B cells. Moreover, the data suggest that the activity of DNA polymerase alpha and that of DNA polymerase delta in B cells might be independently regulated.
语言:: eng
DOI:: 10.1006/cimm.1993.1187

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