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PMID: 8099088 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Categorization of clinical isolates of Helicobacter pylori on the basis of restriction digest analyses of polymerase chain reaction-amplified ureC genes.

Journal of clinical microbiology ·Vol. 31 ·No. 5 ·1993-05-00 ·Pages 1334-5

Moore RA, Kureishi A, Wong S, Bryan LE

Abstract

Restriction endonuclease analyses of a 1.1-kb polymerase chain reaction-amplified portion of the ureC gene from Helicobacter pylori were used to group or to differentiate 21 clinical isolates. Isolates were placed into 4 groups after HindIII digestion alone, and placement was expanded into 15 groups after isolates were digested with AluI and PvuI.

Related Genes

ureC

MeSH Terms

Bacterial Typing Techniques Base Sequence DNA, Bacterial/genetics Genes, Bacterial Helicobacter Infections/microbiology Helicobacter pylori/classification,genetics,isolation & purification Humans Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Urease/genetics

Chemicals

DNA, Bacterial Urease

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Moore R A

Department of Microbiology and Infectious Diseases, University of Calgary, Alberta, Canada.

Kureishi A

Wong S

Bryan L E

References (8)

8 references, click to expand

Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration.
Lancet. 1984 Jun 16;1(8390):1311-5 PMID: 6145023
Restriction endonuclease analysis of the genome of Campylobacter pylori with a rapid extraction method: evidence for considerable genomic variation.
J Infect Dis. 1988 Mar;157(3):465-71 PMID: 2830341
Biotyping of Campylobacter pylori.
J Med Microbiol. 1989 Jul;29(3):203-6 PMID: 2664183
Use of polymerase chain reaction-amplified Helicobacter pylori urease structural genes for differentiation of isolates.
J Clin Microbiol. 1992 Mar;30(3):739-41 PMID: 1313051
Evaluation of techniques for isolation, subcultivation, and preservation of Helicobacter pylori.
J Clin Microbiol. 1991 Jan;29(1):51-3 PMID: 1993767
Shuttle cloning and nucleotide sequences of Helicobacter pylori genes responsible for urease activity.
J Bacteriol. 1991 Mar;173(6):1920-31 PMID: 2001995
Molecular techniques for studying the epidemiology of infection by Helicobacter pylori.
Scand J Gastroenterol Suppl. 1991;181:20-32 PMID: 1866591
Campylobacter pylori: its link to gastritis and peptic ulcer disease.
Rev Infect Dis. 1990 Jan-Feb;12 Suppl 1:S87-93 PMID: 2406862

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.31.5.1334-1335.1993

Published

1993-05-00

Pages

1334-5

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC262931

Subset

External Links

PubMed source DOI: 10.1128/jcm.31.5.1334-1335.1993 PMC Full Text View journal details

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Categorization of clinical isolates of Helicobacter pylori on the basis of restriction digest analyses of polymerase chain reaction-amplified ureC genes.

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