3'-Terminal nucletide sequence (n equals 361) of bacteriophage MS2 RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 7 ·1975-07-00 ·Pages 2559-62

Vandenberghe A, Min Jou W, Fiers W

Abstract

32P-Labeled MS2 RNA was partially digested with ribonuclease T1 (guanyloribonuclease; ribonucleate 3'-guanylo-oligonucleotidohydrolase; EC 3.1.4.8) or with epilson-carboxymethyl-lysine-41 pancreatic ribonuclease A (ribonucleate 3'-pyrimidino-oligonucleotidohydrolase; EC 3.1.4.22). A series of overlapping fragments was obtained which allowed the reconstruction of a 361-nucleotide-long 3'-terminal sequence. A unique reading frame could be deduced, which indicated that the replicase gene ends with a U-A-G termination signal and is followed by a 174-nucleotide-long untranslated segment.

MeSH Terms

Base Sequence Binding Sites Coliphages/analysis Endonucleases Nucleic Acid Conformation Pancreas/enzymology RNA Viruses/analysis RNA, Viral Ribonuclease T1 Ribonucleases Ribonucleotides/analysis

Chemicals

RNA, Viral Ribonucleotides Endonucleases Ribonucleases Ribonuclease T1

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Vandenberghe A

Min Jou W

Fiers W

References (20)

20 references, click to expand

Amino-acid sequence of the "Qbeta" coat protein.
Nature. 1970 Jul 18;227(5255):271-3 PMID: 5428193
Studies on the bacteriophage MS2. XVI. The termination signal of the A protein cistron.
J Mol Biol. 1972 Nov 14;71(2):243-61 PMID: 4564480
A sequence of 83 nucleotides containing the replicase cistron ribosome binding site of phage Qbeta RNA.
Eur J Biochem. 1974 Jan 16;41(2):413-20 PMID: 4593581
Nucleotide sequence of the gene coding for the bacteriophage MS2 coat protein.
Nature. 1972 May 12;237(5350):82-8 PMID: 4555447
Studies on the bacteriophage MS2. IV. The 3'-OH terminal undecanucleotide sequence of the viral RNA chain.
J Mol Biol. 1967 Dec 28;30(3):507-27 PMID: 5598208
Studies on the bacteriophage MS2. An internal nucleotide fragment resembling some ribosomal binding sites.
Eur J Biochem. 1973 Jul 2;36(1):135-43 PMID: 4354616
Structure and function of phage RNA.
Annu Rev Biochem. 1973;42:303-28 PMID: 4581226
Bacteriophage Ms2 RNA: nucleotide sequence of the end of the a protein gene and the intercistronic region.
Nat New Biol. 1973 Jan 24;241(108):99-101 PMID: 4512458
A two-dimensional fractionation procedure for radioactive nucleotides.
J Mol Biol. 1965 Sep;13(2):373-98 PMID: 5325727
Studies on the bacteriophage MS2. The untranslated 5'-terminal nucleotide sequence preceding the first cistron.
Eur J Biochem. 1971 Oct 14;22(3):400-14 PMID: 5125360
Preparative two-dimensional polyacrylamide gel electrophoresis of 32 P-labeled RNA.
Anal Biochem. 1972 Sep;49(1):184-97 PMID: 5081097
Studies on the bacteriophage MS2. IX. The heptanucleotide sequences present in the pancreatic ribonuclease digest of the viral RNA.
J Mol Biol. 1971 May 14;57(3):597-613 PMID: 4325543
Improved estimation of secondary structure in ribonucleic acids.
Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
The 3'-terminal nucleotide sequence (n = 16) of bacteriophage MS2 RNA.
FEBS Lett. 1970 Aug 17;9(4):222-224 PMID: 11947675
Sequence of 51 nucleotides at the 3'-end of R17 bacteriophage RNA.
J Mol Biol. 1972 Jan 14;63(1):41-56 PMID: 5016970
Release factors mediating termination of complete proteins.
Nature. 1970 Jun 13;226(5250):1029-33 PMID: 4911223
The leader sequence from the 5'-terminus to the A-protein initiation codon in MS2-virus RNA.
Proc Natl Acad Sci U S A. 1971 Mar;68(3):585-9 PMID: 5276766
Recent progress in the sequence determination of bacteriophage MS2 RNA.
Biochimie. 1971;53(4):495-506 PMID: 5125256
Studies on the bacteriophage MS2 nucleotide sequence of a 3'-terminal fragment (n = 104).
FEBS Lett. 1971 Oct 15;18(1):141-144 PMID: 11946106
A new method for partial digestion useful for sequence analysis of polynucleotides.
FEBS Lett. 1971 Sep 1;16(4):281-283 PMID: 11945961

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.72.7.2559

Published

1975-07-00

Pages

2559-62

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC432808

Subset

Databases

GENBANK

J02467, V00642

External Links

PubMed source DOI: 10.1073/pnas.72.7.2559 PMC Full Text View journal details

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3'-Terminal nucletide sequence (n equals 361) of bacteriophage MS2 RNA.

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