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PMID: 8096487 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of a lysine-to-glutamic acid mutation in a conservative sequence of farnesyl diphosphate synthase from Saccharomyces cerevisiae.

Gene ·Vol. 125 ·No. 2 ·1993-03-30 ·Pages 185-9

Blanchard L, Karst F

Abstract

The mutant gene erg20-2 was isolated from a yeast strain defective in farnesyl diphosphate synthase (FPPS). This strain had the unusual property of excreting prenyl alcohols such as geraniol. The nucleotide (nt) sequence, compared with that of the wild-type gene, showed a single nt change, resulting in a Lys197-->Glu substitution in FPPS which is directly involved in terpenic alcohol formation. In addition, disruption of ERG20 revealed that in yeast no other prenyl transferase is able to synthesize the FPP molecules required for essential non-sterol metabolites.

MeSH Terms
Acyclic Monoterpenes Alkyl and Aryl Transferases Alleles Amino Acid Sequence Animals Base Sequence Conserved Sequence DNA, Fungal Geranyltranstransferase Glutamates/genetics Glutamic Acid Humans Lysine/genetics Molecular Sequence Data Mutation Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics Sequence Homology, Amino Acid Terpenes/metabolism Transferases/chemistry,genetics
Chemicals
Acyclic Monoterpenes DNA, Fungal Glutamates Terpenes Glutamic Acid Transferases Alkyl and Aryl Transferases Geranyltranstransferase Lysine geraniol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Blanchard L
Laboratoire de Biochimie et Génétique des Microorganismes, Université de Poitiers, France.
Karst F
Article Info
Journal
Gene
Abbr.
Gene
ISSN
0378-1119
DOI
10.1016/0378-1119(93)90326-x
Published
1993-03-30
Pages
185-9
Language
English
Region
Netherlands
NLM ID
7706761
Subset
IM
External Links
PubMed source DOI: 10.1016/0378-1119(93)90326-x View journal details
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