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PMID: 8093697 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

High-level transcription of the major Bacillus subtilis autolysin operon depends on expression of the sigma D gene and is affected by a sin (flaD) mutation.

Journal of bacteriology ·Vol. 175 ·No. 3 ·1993-02-00 ·Pages 795-801

Kuroda A, Sekiguchi J

Abstract

Transcription of the major Bacillus subtilis autolysin gene (cwlB) was investigated. Deletion of the region upstream of the gene cluster lppX-cwbA-cwlB led to a loss of promoter activity. Primer extension analysis suggested that the cwlB operon is transcribed by E sigma D and E sigma A, the former transcripts being predominants at the exponential growth phase. Expression of the lppX-lacZ fusion gene was reduced by about 90% in a sigD-null mutant. A sin (flaD1) mutation caused a severe defect in transcription of the lppX-cwbA-cwlB operon. The sin (flaD1) mutation also reduced expression of a sigD-lacZ fusion gene constructed in the B. subtilis chromosome. Since the sigD-null mutant exhibits motility and autolysin deficiencies and filamentation, similar phenotypes in the sin (flaD1) mutant may be caused by reduction in expression of the sigma D protein.

Related Genes
cwbA cwlB lppX sigD
MeSH Terms
Amino Acid Sequence Bacillus subtilis/genetics Base Sequence Cloning, Molecular DNA-Binding Proteins/genetics Gene Expression Regulation, Bacterial Molecular Sequence Data N-Acetylmuramoyl-L-alanine Amidase/genetics Operon Promoter Regions, Genetic RNA, Messenger/genetics Repressor Proteins/genetics Restriction Mapping Sigma Factor/genetics Transcription, Genetic
Chemicals
DNA-Binding Proteins RNA, Messenger Repressor Proteins Sigma Factor N-Acetylmuramoyl-L-alanine Amidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kuroda A
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
Sekiguchi J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.3.795-801.1993
Published
1993-02-00
Pages
795-801
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC196219
Subset
IM
Databases
GENBANK
D10388
External Links
PubMed source DOI: 10.1128/jb.175.3.795-801.1993 PMC Full Text View journal details
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