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PMID: 8088514 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

SIP1 is a catabolite repression-specific negative regulator of GAL gene expression.

Genetics ·Vol. 137 ·No. 3 ·1994-07-00 ·Pages 689-700

Mylin LM, Bushman VL, Long RM, Yu X, Lebo CM, Blank TE, Hopper JE

Abstract

The yeast Snf1p kinase is required for normal expression of many genes involved in utilization of non-glucose carbon. Snf1p is known to associate with several proteins. One is Sip1p, a protein that becomes phosphorylated in the presence of Snf1p and thus is a candidate Snf1p kinase substrate. We have isolated the SIP1 gene as a multicopy suppressor of the gal83-associated defect in glucose repression of GAL gene expression. Multicopy SIP1 also suppressed the gal82-associated defect in glucose repression, suggesting that SIP1, GAL83 and GAL82 function interdependently. Multicopy SIP1 gene reduces GAL1, GAL2, GAL7 and GAL10 gene expression three- to fourfold in cells grown in the presence of glucose but has no effect in cells grown on nonrepressing carbon. Sip1-deletion cells exhibited a two- to threefold increase in GAL gene expression compared to wild-type cells when grown on glucose. These studies show that SIP1 is a catabolite repression-specific negative regulator of GAL gene expression. Northern analysis revealed two SIP1 transcripts whose relative abundance changed with carbon source. Western blots revealed that Sip1p abundance is not markedly affected by carbon source, suggesting that Sip1p may be regulated post-translationally.

Related Genes
GAL GAL82 GAL83 SIP1
MeSH Terms
AMP-Activated Protein Kinases Base Sequence Carrier Proteins Enzyme Repression/genetics Fungal Proteins/genetics,metabolism Galactokinase/biosynthesis Galactose/metabolism Gene Deletion Gene Expression Regulation, Fungal Genes, Fungal Genes, Suppressor Glucose/metabolism Molecular Sequence Data Multigene Family Protein Kinases/metabolism Protein Serine-Threonine Kinases/metabolism Repressor Proteins/genetics Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins Signal Transduction/genetics Substrate Specificity Transcription Factors/metabolism
Chemicals
Carrier Proteins Fungal Proteins GAL83 protein, S cerevisiae Repressor Proteins Saccharomyces cerevisiae Proteins Transcription Factors Protein Kinases SNF1-related protein kinases Galactokinase Protein Serine-Threonine Kinases SIP1 protein, S cerevisiae SNF4 protein, S cerevisiae AMP-Activated Protein Kinases Glucose Galactose
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Mylin L M
Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey 17033.
Bushman V L
Long R M
Yu X
Lebo C M
Blank T E
Hopper J E
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/137.3.689
Published
1994-07-00
Pages
689-700
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1206028
Subset
IM
Grants
NIGMS NIH HHS · GM 27925 · United States
External Links
PubMed source DOI: 10.1093/genetics/137.3.689 PMC Full Text View journal details
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