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PMID: 8084017 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Recombinant rabbit hemorrhagic disease virus capsid protein expressed in baculovirus self-assembles into viruslike particles and induces protection.

Journal of virology ·Vol. 68 ·No. 10 ·1994-10-00 ·Pages 6794-8

Laurent S, Vautherot JF, Madelaine MF, Le Gall G, Rasschaert D

Abstract

VP60, the unique component of rabbit hemorrhagic disease virus capsid, was expressed in the baculovirus system. The recombinant VP60, released in the supernatant of infected insect cells, assembled without the need of any other viral component to form viruslike particles (VLPs), structurally and immunologically indistinguishable from the rabbit hemorrhagic disease virion. Intramuscular vaccination of rabbits with the VLPs conferred complete protection in 15 days; this protection was found to be effective from the fifth day after VLP injection and was accompanied by a strong humoral response.

MeSH Terms
Amino Acid Sequence Animals Baculoviridae Base Sequence Caliciviridae Infections/microbiology,pathology Capsid/biosynthesis,immunology Cell Line Genetic Vectors Hemorrhagic Disease Virus, Rabbit/isolation & purification,metabolism,ultrastructure Kinetics Liver/microbiology,ultrastructure Microscopy, Electron Molecular Sequence Data Moths Rabbits Recombinant Proteins/biosynthesis Time Factors Vaccines, Synthetic/immunology Viral Vaccines/immunology Virion/isolation & purification,metabolism,ultrastructure
Chemicals
Recombinant Proteins Vaccines, Synthetic Viral Vaccines
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Laurent S
Unité de Virologie et d'Immunologie Moléculaires, Institut National de la Recherche Agronomique, Jouy en Josas, France.
Vautherot J F
Madelaine M F
Le Gall G
Rasschaert D
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.10.6794-6798.1994
Published
1994-10-00
Pages
6794-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC237106
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.68.10.6794-6798.1994 PMC Full Text View journal details
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