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PMID: 807681 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Properties of some norvaline-resistant mutants of Bacillus subtilis.

Journal of general microbiology ·Vol. 88 ·No. 2 ·1975-06-00 ·Pages 289-94

Holtzclaw WD, Chapman LF

Abstract

DL-Norvaline inhibits growth of wild-type Bacillus subtilis. A number of mutants resistant to growth inhibition by this analogue were isolated and studied. Cross-feeding experiments and paper chromatography of culture supernatants indicated that the mutants excreted leucine and possibly valine and glutamate. Enzymic analysis indicated that the mutants were derepressed for acetohydroxy-acid synthetase and alpha-isopropylmalate synthetase; however, no derepression of threonine deaminase, dihydroxyacid dehydrase or transaminase B was observed.

MeSH Terms

2-Isopropylmalate Synthase/metabolism Acetolactate Synthase/metabolism Bacillus subtilis/enzymology,metabolism Chromosome Mapping Culture Media Drug Resistance, Microbial Enzyme Repression Genes Genetic Linkage Glutamates/biosynthesis Hydro-Lyases/metabolism Isomerism Leucine/biosynthesis Mutation Operon Threonine Dehydratase/metabolism Transaminases/metabolism Transformation, Genetic Valine/biosynthesis,pharmacology

Chemicals

Culture Media Glutamates Acetolactate Synthase 2-Isopropylmalate Synthase Transaminases Hydro-Lyases Threonine Dehydratase Leucine Valine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Holtzclaw W D

Chapman L F

Article Info

Journal

Journal of general microbiology

Abbr.

J Gen Microbiol

ISSN

0022-1287

DOI

10.1099/00221287-88-2-289

Published

1975-06-00

Pages

289-94

Language

English

Region

England

NLM ID

0375371

Subset

External Links

PubMed source DOI: 10.1099/00221287-88-2-289

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Properties of some norvaline-resistant mutants of Bacillus subtilis.

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