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PMID: 8075538 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

1H and 15N resonance assignments and secondary structure of the carbon monoxide complex of sperm whale myoglobin.

Journal of biomolecular NMR ·Vol. 4 ·No. 4 ·1994-07-00 ·Pages 491-504

Thériault Y, Pochapsky TC, Dalvit C, Chiu ML, Sligar SG, Wright PE

Abstract

Sequence-specific backbone 1H and 15N resonance assignments have been made for 95% of the amino acids in sperm whale myoglobin, complexed with carbon monoxide (MbCO). Many assignments for side-chain resonances have also been obtained. Assignments were made by analysis of an extensive series of homonuclear 2D spectra, measured with unlabeled protein, and both 2D and 3D 1H-15N-correlated spectra obtained from uniformly 15N-labeled myoglobin. Patterns of medium-range NOE connectivities indicate the presence of eight helices in positions that are very similar to those found in the crystal structures of sperm whale myoglobin. The resonance assignments of MbCO form the basis for determination of the solution structure and for hydrogen-exchange measurements to probe the stability and folding pathways of myoglobin. They will also form a basis for assignment of the spectra of single-site mutants with altered ligand-binding properties.

MeSH Terms
Amino Acid Sequence Animals Carbon Monoxide/chemistry Hydrogen Magnetic Resonance Spectroscopy Molecular Sequence Data Myoglobin/chemistry Nitrogen Isotopes Protein Structure, Secondary Recombinant Fusion Proteins/chemistry Whales/blood
Chemicals
Myoglobin Nitrogen Isotopes Recombinant Fusion Proteins carboxymyoglobin Carbon Monoxide Hydrogen
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Thériault Y
Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037.
Pochapsky T C
Dalvit C
Chiu M L
Sligar S G
Wright P E
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Article Info
Journal
Journal of biomolecular NMR
Abbr.
J Biomol NMR
ISSN
0925-2738
DOI
10.1007/BF00156616
Published
1994-07-00
Pages
491-504
Language
English
Region
Netherlands
NLM ID
9110829
Subset
IM
Grants
NIDDK NIH HHS · DK34909 · United States
NIGMS NIH HHS · GM31756 · United States
External Links
PubMed source DOI: 10.1007/BF00156616 View journal details
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