Home LiteratureArticle Details
PMID: 8065929 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

LRG1 is expressed during sporulation in Saccharomyces cerevisiae and contains motifs similar to LIM and rho/racGAP domains.

Nucleic acids research ·Vol. 22 ·No. 15 ·1994-08-11 ·Pages 3151-4

Müller L, Xu G, Wells R, Hollenberg CP, Piepersberg W

Abstract

We here report the sequence of a yeast gene LRG1 whose deduced amino acid sequence contains sequence motifs similar to LIM domain proteins in the amino-terminal, and to rho/rac GTPase activating proteins (rho/racGAP's) in the carboxy-terminal part. LRG1 expression is differentially regulated showing a peak of expression in sporulating cells. Gene disruption experiments indicate that the gene is not essential and may play a role during mating.

Related Genes
LRG1
MeSH Terms
Amino Acid Sequence Base Sequence DNA, Fungal/chemistry,isolation & purification DNA-Binding Proteins/chemistry,genetics GTP-Binding Proteins/chemistry GTPase-Activating Proteins Gene Expression Genes, Fungal Molecular Sequence Data Saccharomyces cerevisiae/genetics,physiology Saccharomyces cerevisiae Proteins Sequence Homology Spores, Fungal/physiology rac GTP-Binding Proteins
Chemicals
DNA, Fungal DNA-Binding Proteins GTPase-Activating Proteins LRG1 protein, S cerevisiae Saccharomyces cerevisiae Proteins GTP-Binding Proteins rac GTP-Binding Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Müller L
German Cancer Research Center, Heidelberg.
Xu G
Wells R
Hollenberg C P
Piepersberg W
References (18)
18 references, click to expand
  1. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  2. Physical map of the Saccharomyces cerevisiae genome at 110-kilobase resolution.
    Genetics. 1991 Apr;127(4):681-98 PMID: 2029969
  3. Developmental regulation of a gene that encodes a cysteine-rich intestinal protein and maps near the murine immunoglobulin heavy chain locus.
    Proc Natl Acad Sci U S A. 1986 Apr;83(8):2516-20 PMID: 3085096
  4. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions.
    Gene. 1986;45(3):299-310 PMID: 3026915
  5. Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein.
    Mol Cell Biol. 1987 Jul;7(7):2316-28 PMID: 3302672
  6. bcr genes and transcripts.
    Oncogene. 1988 Feb;2(2):113-7 PMID: 3285291
  7. mec-3, a homeobox-containing gene that specifies differentiation of the touch receptor neurons in C. elegans.
    Cell. 1988 Jul 1;54(1):5-16 PMID: 2898300
  8. Novel human brain cDNA encoding a 34,000 Mr protein n-chimaerin, related to both the regulatory domain of protein kinase C and BCR, the product of the breakpoint cluster region gene.
    J Mol Biol. 1990 Jan 5;211(1):11-6 PMID: 2299665
  9. The LIM region of a presumptive Caenorhabditis elegans transcription factor is an iron-sulfur- and zinc-containing metallodomain.
    Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9210-3 PMID: 1924383
  10. An efficient transformation procedure enabling long-term storage of competent cells of various yeast genera.
    Yeast. 1991 Oct;7(7):691-2 PMID: 1776359
  11. The rhombotin gene family encode related LIM-domain proteins whose differing expression suggests multiple roles in mouse development.
    J Mol Biol. 1992 Aug 5;226(3):747-61 PMID: 1507224
  12. Zyxin and cCRP: two interactive LIM domain proteins associated with the cytoskeleton.
    J Cell Biol. 1992 Dec;119(6):1573-87 PMID: 1469049
  13. Proteins regulating Ras and its relatives.
    Nature. 1993 Dec 16;366(6456):643-54 PMID: 8259209
  14. rho family GTPase activating proteins p190, bcr and rhoGAP show distinct specificities in vitro and in vivo.
    EMBO J. 1993 Dec 15;12(13):5151-60 PMID: 8262058
  15. Mutational analysis of the metal sites in an LIM domain.
    J Biol Chem. 1994 Apr 15;269(15):11108-13 PMID: 8157637
  16. FUS3 encodes a cdc2+/CDC28-related kinase required for the transition from mitosis into conjugation.
    Cell. 1990 Feb 23;60(4):649-64 PMID: 2406028
  17. Novel cysteine-rich motif and homeodomain in the product of the Caenorhabditis elegans cell lineage gene lin-11.
    Nature. 1990 Apr 26;344(6269):876-9 PMID: 1970421
  18. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.15.3151
Published
1994-08-11
Pages
3151-4
Language
English
Region
England
NLM ID
0411011
PMCID
PMC310289
Subset
IM
Databases
GENBANK
X78453
External Links
PubMed source DOI: 10.1093/nar/22.15.3151 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2