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PMID: 8061203 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Kinetic mechanism of myofibril ATPase.

Biophysical journal ·Vol. 66 ·No. 5 ·1994-05-00 ·Pages 1542-53

Ma YZ, Taylor EW

Abstract

The kinetic mechanism of myofibril ATPase was investigated using psoas and mixed back muscle over a range of ionic strengths. Myofibrils were labeled with pyrene iodoacetamide to measure the rate constants for the binding of ATP and formation of the weakly attached state. The velocity of shortening was measured by stopping the contraction at various times by mixing with pH 4.5 buffer. The transient and steady-state rates of ATP hydrolysis were measured by the quench flow method. The results fitted the kinetic scheme [formula: see text] The rate constants (or equilibrium constants for steps 1 and 6) were obtained for the six steps. k5 was calculated from the KM for shortening velocity, K1, and k2. The rate constants were essentially equal for myofibrils and acto-S-1 at low ionic strength. Increasing the ionic strength up to 100 mM in NaCl increased the rate of the hydrolysis step and the size of the phosphate burst and the effective rate of product release became the rate-limiting step. The step calculated from the velocity of shortening, k5, and k2 is 15 nm, based on a model in which step 4 is the force-generating step.

MeSH Terms
Adenosine Diphosphate/pharmacology Adenosine Triphosphate/metabolism Animals Biophysical Phenomena Biophysics Hydrolysis In Vitro Techniques Kinetics Muscle Contraction/physiology Myofibrils/enzymology Myosins/antagonists & inhibitors,metabolism Phosphates/metabolism,pharmacology Pyrenes
Chemicals
Phosphates Pyrenes Adenosine Diphosphate Adenosine Triphosphate pyrene Myosins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ma Y Z
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Taylor E W
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(94)80945-2
Published
1994-05-00
Pages
1542-53
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1275874
Subset
IM
Grants
NHLBI NIH HHS · HL 20592 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(94)80945-2 PMC Full Text View journal details
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