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PMID: 8052657 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Complexes between STE5 and components of the pheromone-responsive mitogen-activated protein kinase module.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 16 ·1994-08-02 ·Pages 7762-6

Marcus S, Polverino A, Barr M, Wigler M

Abstract

We present genetic evidence for complex formation of STE5 and the STE11, STE7, and FUS3 protein kinases, the pheromone-responsive mitogen-activated protein kinase module of Saccharomyces cerevisiae. Interaction between STE5 and STE11 is not dependent on STE7, and interaction between STE5 and STE7 does not require STE11. The N-terminal regulatory domain of STE11 is both necessary and sufficient for interaction with STE5. Interaction between STE7 and STE11 is bridged by STE5, suggesting the formation of a multiprotein complex. We also demonstrate biochemical interaction between STE5 and STE11 by using a combination of bacterially expressed fusion proteins and extracts prepared from yeast. Our results suggest that STE5 is a scaffolding protein that facilitates interactions between components of the pheromone-responsive mitogen-activated protein kinase module. We further propose that such scaffolding proteins serve to inhibit cross-talk between functionally unrelated mitogen-activated protein kinase modules within the same cell.

MeSH Terms
Adaptor Proteins, Signal Transducing Calcium-Calmodulin-Dependent Protein Kinases/genetics,metabolism Carrier Proteins Fungal Proteins/genetics,metabolism MAP Kinase Kinase 1 MAP Kinase Kinase Kinases/metabolism Mitogen-Activated Protein Kinase Kinases Mitogen-Activated Protein Kinases Pheromones/metabolism Protein Kinases/genetics,metabolism Protein Serine-Threonine Kinases/metabolism Protein-Tyrosine Kinases/metabolism Saccharomyces cerevisiae/physiology Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Signal Transduction Transcription Factors
Chemicals
Adaptor Proteins, Signal Transducing Carrier Proteins Fungal Proteins Pheromones STE5 protein, S cerevisiae Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Transcription Factors ste11 protein, S pombe Protein Kinases byr1 protein kinase Protein-Tyrosine Kinases Protein Serine-Threonine Kinases Calcium-Calmodulin-Dependent Protein Kinases FUS3 protein, S cerevisiae Mitogen-Activated Protein Kinases MAP Kinase Kinase Kinases Ste11 protein, S cerevisiae MAP Kinase Kinase 1 Mitogen-Activated Protein Kinase Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Marcus S
Cold Spring Harbor Laboratory, NY 11724.
Polverino A
Barr M
Wigler M
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47 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.16.7762
Published
1994-08-02
Pages
7762-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44482
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.91.16.7762 PMC Full Text View journal details
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