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PMID: 8052645 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Lasting potentiation of inhibition is associated with an increased number of gamma-aminobutyric acid type A receptors activated during miniature inhibitory postsynaptic currents.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 16 ·1994-08-02 ·Pages 7698-702

Otis TS, De Koninck Y, Mody I

Abstract

Whole-cell patch-clamp recordings unveiled a substantial increase in the amplitude, but no change in the frequency, of miniature inhibitory postsynaptic currents (mIPSCs) in dentate gyrus granule cells following chronic epilepsy induced by kindling. This novel and persistent enhancement of gamma-aminobutyric acid type A (GABAA) receptor-mediated inhibition lasted for at least 48 hr following its induction. Nearly a doubling of the number of activated functional postsynaptic GABAA receptor channels during mIPSCs without any change in single-channel conductance or kinetics could be demonstrated using nonstationary fluctuation analysis. As postsynaptic GABAA receptors are likely to be pharmacologically saturated by the transmitter concentration in the cleft, incrementing the number of functional receptor channels may be the most effective means to augment inhibition in the mammalian brain.

MeSH Terms
Animals Chronic Disease Disease Models, Animal Electric Conductivity Epilepsy/metabolism Hippocampus/physiology Kindling, Neurologic/physiology Long-Term Potentiation/physiology Male Rats Rats, Wistar Receptors, GABA-A/physiology Synapses/physiology
Chemicals
Receptors, GABA-A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Otis T S
Department of Anesthesiology and Pain Management, University of Texas Southwestern Medical Center, Dallas 75235-9068.
De Koninck Y
Mody I
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45 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.16.7698
Published
1994-08-02
Pages
7698-702
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44469
Subset
IM
Grants
NINDS NIH HHS · NS-12151 · United States
NINDS NIH HHS · NS-27528 · United States
NINDS NIH HHS · NS-30549 · United States
External Links
PubMed source DOI: 10.1073/pnas.91.16.7698 PMC Full Text View journal details
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