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PMID: 8051002 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

New osmoregulated beta(1-3),beta(1-6) glucosyltransferase(s) in Azospirillum brasilense.

Journal of bacteriology ·Vol. 176 ·No. 16 ·1994-08-00 ·Pages 4890-8

Altabe SG, Iñón de Iannino N, de Mendoza D, Ugalde RA

Abstract

A linear beta(1-3),beta(1-6) glucan was detected in the periplasm of Azospirillum brasilense cells growing in a medium of low osmotic strength. This glucan was produced in vitro by purified bacterial inner membranes with UDP-glucose as the sugar donor in the presence of Mg2+. Growth in a high-osmotic-strength medium strongly reduced the amount of this glucan accumulated in the periplasmic space, and the inhibition was associated with a reduction in the enzymatic activity of the beta(1-3),beta(1-6) glucosyltransferase(s).

MeSH Terms

Azospirillum brasilense/enzymology Cell Compartmentation Glucans/biosynthesis,chemistry Glucosyltransferases/metabolism Magnetic Resonance Spectroscopy Periodic Acid/chemistry Water-Electrolyte Balance

Chemicals

Glucans Periodic Acid Glucosyltransferases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Altabe S G

Instituto de Investigaciones Bioquímicas Fundación Campomar, Buenos Aires, Argentina.

Iñón de Iannino N

de Mendoza D

Ugalde R A

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.176.16.4890-4898.1994

Published

1994-08-00

Pages

4890-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC196324

Subset

External Links

PubMed source DOI: 10.1128/jb.176.16.4890-4898.1994 PMC Full Text View journal details

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New osmoregulated beta(1-3),beta(1-6) glucosyltransferase(s) in Azospirillum brasilense.

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