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PMID: 8041738 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Cloning and sequencing of thiol-specific antioxidant from mammalian brain: alkyl hydroperoxide reductase and thiol-specific antioxidant define a large family of antioxidant enzymes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 15 ·1994-07-19 ·Pages 7017-21

Chae HZ, Robison K, Poole LB, Church G, Storz G, Rhee SG

Abstract

A cDNA corresponding to a thiol-specific antioxidant enzyme (TSA) was isolated from a rat brain cDNA library with the use of antibodies to bovine TSA. The cDNA clone encoded an open reading frame capable of encoding a 198-residue polypeptide. The rat and yeast TSA proteins show significant sequence homology to the 21-kDa component (AhpC) of Salmonella typhimurium alkyl hydroperoxide reductase, and we have found that AhpC exhibits TSA activity. AhpC and TSA define a family of > 25 different proteins present in organisms from all kingdoms. The similarity among the family members extends over the entire sequence and ranges between 23% and 98% identity. A majority of the members of the AhpC/TSA family contain two conserved cysteines. At least eight of the genes encoding AhpC/TSA-like polypeptides are found in proximity to genes encoding other oxidoreductase activities, and the expression of several of the homologs has been correlated with pathogenicity. We suggest that the AhpC/TSA family represents a widely distributed class of antioxidant enzymes. We also report that a second family of proteins, defined by the 57-kDa component (AhpF) of alkyl hydroperoxide reductase and by thioredoxin reductase, has expanded to include six additional members.

MeSH Terms
Amino Acid Sequence Animals Antioxidants/chemistry,metabolism Base Sequence Brain/enzymology Cattle Cloning, Molecular DNA Humans Molecular Sequence Data Oxidoreductases/chemistry,metabolism Peroxidases Peroxiredoxins Proteins/chemistry,metabolism Rats Sequence Homology, Amino Acid
Chemicals
Antioxidants Proteins DNA Oxidoreductases Peroxidases Peroxiredoxins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Chae H Z
Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
Robison K
Poole L B
Church G
Storz G
Rhee S G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.15.7017
Published
1994-07-19
Pages
7017-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44329
Subset
IM
Grants
NIGMS NIH HHS · R01 GM050389 · United States
Databases
GENBANK
U06099
External Links
PubMed source DOI: 10.1073/pnas.91.15.7017 PMC Full Text View journal details
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