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PMID: 8041605 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The transcription factor YY1 binds to negative regulatory elements in the human cytomegalovirus major immediate early enhancer/promoter and mediates repression in non-permissive cells.

Nucleic acids research ·Vol. 22 ·No. 13 ·1994-07-11 ·Pages 2453-9

Liu R, Baillie J, Sissons JG, Sinclair JH

Abstract

We have previously shown that repression of human cytomegalovirus (HCMV) major immediate early (IE) gene expression in non-permissive human teratocarcinoma (T2) cells is associated with a number of nuclear factors which bind to the imperfect dyad symmetry located in the modulator region upstream of the major IE enhancer as well as to the 21 bp repeat elements within the enhancer. Differentiation of T2 cells with retinoic acid (RA) results in a decrease in binding of some of these nuclear factors to these sites and deletion of these specific binding sites from major IE promoter/reporter constructs results in increased IE promoter activity in normally non-permissive cells. In this study, we demonstrate that the transcription factor YY1, which can negatively regulate the adeno-associated virus P5 promoter, directly binds to both the imperfect dyad symmetry and the 21 bp repeat elements in the HCMV major IE promoter/regulatory region and mediates repression of HCMV IE gene expression. This strongly suggests that YY1 plays an important role in regulating HCMV expression in non-permissive cells.

MeSH Terms
Antigens, Viral/genetics Base Sequence Binding Sites Blotting, Western Cell Differentiation Cloning, Molecular Cytomegalovirus/genetics DNA, Viral DNA-Binding Proteins/metabolism Enhancer Elements, Genetic Erythroid-Specific DNA-Binding Factors Gene Expression Regulation, Viral Humans Immediate-Early Proteins/genetics Molecular Sequence Data Promoter Regions, Genetic Repetitive Sequences, Nucleic Acid Transcription Factors/metabolism Tretinoin/pharmacology Tumor Cells, Cultured YY1 Transcription Factor
Chemicals
Antigens, Viral DNA, Viral DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors Immediate-Early Proteins Transcription Factors YY1 Transcription Factor YY1 protein, human immediate-early proteins, cytomegalovirus Tretinoin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Liu R
Department of Medicine, University of Cambridge, Addenbrooke's Hospital, UK.
Baillie J
Sissons J G
Sinclair J H
References (39)
39 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.13.2453
Published
1994-07-11
Pages
2453-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC308195
Subset
IM
Grants
Medical Research Council · G9202171 · United Kingdom
External Links
PubMed source DOI: 10.1093/nar/22.13.2453 PMC Full Text View journal details
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