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PMID: 8035530 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Specificity and sequence requirements for interactions between various retroviral Gag proteins.

Journal of virology ·Vol. 68 ·No. 8 ·1994-08-00 ·Pages 5300-5

Franke EK, Yuan HE, Bossolt KL, Goff SP, Luban J

Abstract

We previously established a genetic assay for retroviral Gag polyprotein multimerization (J. Luban, K. B. Alin, K. L. Bossolt, T. Humaran, and S. P. Goff, J. Virol. 66:5157-5160, 1992). Here we use this assay to demonstrate homomeric interactions between Gag polyproteins encoded by six different retroviruses. Of the Gag polyproteins tested, only those encoded by closely related retroviruses formed heteromultimers. To determine the primary sequence requirements for human immunodeficiency virus type 1 Gag polyprotein multimerization, we studied the effects on multimerization of deletion and linker insertion mutations. Sequences necessary for this process were located between the C-terminal one-third of the capsid domain and the C terminus of the nucleocapsid domain.

MeSH Terms
Animals Base Sequence DNA, Viral Gene Products, gag/genetics,metabolism Genetic Techniques Humans Molecular Sequence Data Mutagenesis, Insertional Retroviridae/genetics,metabolism
Chemicals
DNA, Viral Gene Products, gag
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Franke E K
Department of Medicine, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
Yuan H E
Bossolt K L
Goff S P
Luban J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.8.5300-5305.1994
Published
1994-08-00
Pages
5300-5
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC236479
Subset
IM
Grants
NIAID NIH HHS · NIAID AI 00988 · United States
NIAID NIH HHS · NIAID AI 24845 · United States
External Links
PubMed source DOI: 10.1128/JVI.68.8.5300-5305.1994 PMC Full Text View journal details
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