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PMID: 8035493 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nuclear localization of foamy virus Gag precursor protein.

Journal of virology ·Vol. 68 ·No. 8 ·1994-08-00 ·Pages 4946-54

Schliephake AW, Rethwilm A

Abstract

All foamy viruses give rise to a strong nuclear staining when infected cells are reacted with sera from infected hosts. This nuclear fluorescence distinguishes foamy viruses from all other retroviruses. The experiments reported here indicate that the foamy virus Gag precursor protein is transiently located in the nuclei of infected cells and this is the likely reason for the typical foamy virus nuclear fluorescence. By using the vaccinia virus expression system, a conserved basic sequence motif in the nucleocapsid domain of foamy virus Gag proteins was identified to be responsible for the nuclear transport of the gag precursor molecule. This motif was also found to be able to direct a heterologous protein, the Gag protein of human immunodeficiency virus, into the nucleus.

MeSH Terms

Amino Acid Sequence Animals Biological Transport Cell Line Cell Nucleus/metabolism Chlorocebus aethiops Cricetinae Fluorescent Antibody Technique Gene Products, gag/metabolism Humans Mice Molecular Sequence Data Protein Precursors/metabolism Sequence Homology, Amino Acid Spumavirus/genetics,physiology Tumor Cells, Cultured Vero Cells Virus Replication

Chemicals

Gene Products, gag Protein Precursors

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Schliephake A W

Institut für Virologie und Immunbiologie, Würzburg, Germany.

Rethwilm A

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.68.8.4946-4954.1994

Published

1994-08-00

Pages

4946-54

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC236435

Subset

External Links

PubMed source DOI: 10.1128/JVI.68.8.4946-4954.1994 PMC Full Text View journal details

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Nuclear localization of foamy virus Gag precursor protein.

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