Genetic analysis and overexpression of lipolytic activity in Bacillus subtilis.

Applied and environmental microbiology ·Vol. 60 ·No. 5 ·1994-05-00 ·Pages 1670-3

Dartois V, Coppée JY, Colson C, Baulard A

Abstract

The previously cloned Bacillus subtilis lipase gene (lip) was mapped on the chromosome and used in the construction of a B. subtilis derivative totally devoid of any lip sequence. Homologous overexpression was performed in this strain by subcloning the lip open reading frame on a multicopy plasmid under the control of a strong gram-positive promoter. A 100-fold overproducing strain was obtained, which should facilitate purification of the secreted protein. Furthermore, the delta lip strain BCL1050 constitutes an ideal host for the cloning of heterologous lipase genes.

Related Genes

lip

MeSH Terms

Alleles Bacillus subtilis/enzymology,genetics Chromosome Mapping Chromosomes, Bacterial Gene Expression Lipase/biosynthesis,genetics,metabolism Plasmids

Chemicals

Lipase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Dartois V

Laboratoire de Génétique Microbienne, Université Catholique de Louvain, Belgium.

Coppée J Y

Colson C

Baulard A

References (13)

13 references, click to expand

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.60.5.1670-1673.1994

Published

1994-05-00

Pages

1670-3

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC201535

Subset

External Links

PubMed source DOI: 10.1128/aem.60.5.1670-1673.1994 PMC Full Text View journal details

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Genetic analysis and overexpression of lipolytic activity in Bacillus subtilis.

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