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PMID: 8016118 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

N-arginine dibasic convertase, a metalloendopeptidase as a prototype of a class of processing enzymes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 13 ·1994-06-21 ·Pages 6078-82

Pierotti AR, Prat A, Chesneau V, Gaudoux F, Leseney AM, Foulon T, Cohen P

Abstract

N-Arg dibasic convertase is a metalloendopeptidase from rat brain cortex and testis that cleaves peptide substrates on the N terminus of Arg residues in dibasic stretches. By using both an oligonucleotide and antibodies to screen a rat testis cDNA library, a full-length cDNA was isolated. The sequence contains an open reading frame of 1161 codons corresponding to a protein of 133 kDa that exhibits 35% and 48% similarity with Escherichia coli protease III (pitrilysin, EC 3.4.99.44) and rat or human insulinase (EC 3.4.99.45), respectively. Moreover, the presence of the HXXEH amino acid signature (XX = FL) clearly classifies N-Arg dibasic convertase as a member of the pitrilysin family of zinc-metalloendopeptidases. In addition, a Cys residue that may be responsible for the thiol sensitivity of the insulinase and N-Arg dibasic convertase was proposed. The protein sequence contains a distinctive additional feature consisting of a stretch of 71 acidic amino acids. We hypothesize that this metalloendopeptidase may be a member of a distinct class of processing enzymes.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cerebral Cortex/enzymology Conserved Sequence Endopeptidases/genetics Escherichia coli/enzymology,genetics Gene Library In Situ Hybridization Male Metalloendopeptidases/biosynthesis,genetics,metabolism Molecular Sequence Data Oligodeoxyribonucleotides Operon Protein Processing, Post-Translational Rats Rats, Wistar Sequence Homology, Amino Acid Testis/enzymology
Chemicals
Oligodeoxyribonucleotides Endopeptidases Metalloendopeptidases pitrilysin nardilysin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Pierotti A R
Université Pierre et Marie Curie, Unité de Recherches Associée au Centre National de la Recherche Scientifique, Paris, France.
Prat A
Chesneau V
Gaudoux F
Leseney A M
Foulon T
Cohen P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.13.6078
Published
1994-06-21
Pages
6078-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44141
Subset
IM
Databases
GENBANK
L27124
External Links
PubMed source DOI: 10.1073/pnas.91.13.6078 PMC Full Text View journal details
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