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PMID: 8011235 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular phylogeny of part of the env gene of HIV-1 strains isolated in Côte d'Ivoire.

AIDS (London, England) ·Vol. 8 ·No. 1 ·1994-01-00 ·Pages 21-6

Janssens W, Heyndrickx L, Van de Peer Y, Bouckaert A, Fransen K, Motte J, Gershy-Damet GM, Peeters M, Piot P, van der Groen G

Abstract

To examine the genetic variation of HIV-1 isolates in Abidjan, Côte d'Ivoire, and to determine the extent to which phylogenetic trees based on sequence information of part of the env gene containing the principal neutralizing domain are representative for documenting genetic variability. Phylogenetic comparison of 13 HIV-1 strains isolated from patients in Abidjan with previously documented HIV-1 strains of different geographic origin. To sequence a 900 base-pair fragment of the env gene containing V3, V4, V5 and the beginning of gp41 of three to four clones per isolate. Phylogenetic tree analysis was performed with the software package TREECON. Eleven HIV-1 isolates of Abidjan were classified as genotype A, while two were classed as genotypes B and D. Intra-genotype A distances at the nucleotide level were a maximum of 14.1%. Inter-genotype distances between genotype A and genotypes B, C, and D varied from 16.0 to 22.6%. Phylogenetic trees, based on sequence data of a 300 base-pair fragment containing the V3 loop, showed significant differences in tree topology and statistical confidence with phylogenetic trees based on sequence data of the 900 base-pair env fragment. Genotype A Côte d'Ivoire HIV-1 strains, which comprise 11 out of 13 isolates, predominate in Abidjan, which may indicate a local burst of particular variants. Phylogenetic trees should be interpreted with caution when based on a more limited number of nucleotides, such as the V3 region.

Related Genes

env

MeSH Terms

Base Sequence Cote d'Ivoire DNA, Viral Genes, env Genetic Variation HIV Envelope Protein gp120/genetics HIV Infections/microbiology HIV-1/classification,genetics,isolation & purification Humans Molecular Sequence Data Peptide Fragments/genetics Phylogeny

Chemicals

DNA, Viral HIV Envelope Protein gp120 HIV envelope protein gp120 (305-321) Peptide Fragments

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Janssens W

Department of Infection and Immunity, University of Antwerp, Belgium.

Heyndrickx L

Van de Peer Y

Bouckaert A

Fransen K

Motte J

Gershy-Damet G M

Peeters M

Piot P

van der Groen G

Article Info

Journal

AIDS (London, England)

Abbr.

AIDS

ISSN

0269-9370

DOI

10.1097/00002030-199401000-00004

Published

1994-01-00

Pages

21-6

Language

English

Region

England

NLM ID

8710219

Subset

Databases

GENBANK

X72024, X72025, X72026, X72027, X72028, X72029, X72030, X72031, X72032, X72033, X72034, X72035, X72036, X72037, X72038, X72039, X72040, X72041, X72042, X72043, X72044, X72045, X72046, X72047, X72048, X72049, X72050, X72051, X72052, X72053

External Links

PubMed source DOI: 10.1097/00002030-199401000-00004 View journal details

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Molecular phylogeny of part of the env gene of HIV-1 strains isolated in Côte d'Ivoire.

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