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PMID: 8002609 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Bacillus subtilis rsbU gene product is necessary for RsbX-dependent regulation of sigma B.

Journal of bacteriology ·Vol. 177 ·No. 1 ·1995-01-00 ·Pages 114-22

Voelker U, Dufour A, Haldenwang WG

Abstract

sigma B is a secondary sigma factor of Bacillus subtilis. sigma B-dependent transcription is induced when B. subtilis enters the stationary phase of growth or is exposed to any of a number of different environmental stresses. Three genes (rsbV, rsbW, and rsbX), which are cotranscribed with the sigma B structural gene (sigB), encode regulators of sigma B-dependent gene expression. RsbW and RsbV have been shown to control sigma B activity, functioning as an inhibitory sigma B binding protein and its antagonist, respectively. Using the SPAC promoter (PSPAC) to control the expression of the sigB operon, a ctc::lacZ reporter system to monitor sigma B activity, and monoclonal antibodies to determine the levels of sigB operon products, we have now obtained evidence that RsbX is an indirect regulator of sigma B activity. Genetic data and in vivo measurements argue that RsbX negatively regulates an extension of the RsbV-RsbW pathway that requires the product of an additional regulatory gene (rsbU) which lies immediately upstream of the sigB operon. The results are consistent with RsbU, or a process dependent on RsbU, being able to facilitate the RsbV-dependent release of sigma B from RsbW but normally prevented from doing this by RsbX.

Related Genes
rsbU rsbX sigB
MeSH Terms
Amino Acid Sequence Bacillus subtilis/genetics,growth & development Bacterial Proteins/biosynthesis,genetics Base Sequence Gene Expression Regulation, Bacterial Genes, Bacterial/genetics Genes, Regulator/genetics Kanamycin Resistance Models, Genetic Molecular Sequence Data Operon/genetics Phosphoric Monoester Hydrolases Promoter Regions, Genetic/genetics Recombinant Fusion Proteins/biosynthesis Sequence Deletion Sigma Factor/biosynthesis Transcription, Genetic
Chemicals
Bacterial Proteins Recombinant Fusion Proteins SigB protein, Bacteria Sigma Factor Phosphoric Monoester Hydrolases RsbU protein, Bacillus subtilis
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Voelker U
Department of Microbiology, University of Texas Health Science Center at San Antonio 78284-7758.
Dufour A
Haldenwang W G
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27 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.1.114-122.1995
Published
1995-01-00
Pages
114-22
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC176563
Subset
IM
Grants
NIGMS NIH HHS · R01 GM48220 · United States
Databases
GENBANK
X81652
External Links
PubMed source DOI: 10.1128/jb.177.1.114-122.1995 PMC Full Text View journal details
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