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PMID: 7988290 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The nuclear position of pericentromeric DNA of chromosome 11 appears to be random in G0 and non-random in G1 human lymphocytes.

Chromosoma ·Vol. 103 ·No. 4 ·1994-07-00 ·Pages 286-92

Hulspas R, Houtsmuller AB, Krijtenburg PJ, Bauman JG, Nanninga N

Abstract

The nuclear topography of pericentromeric DNA of chromosome 11 was analyzed in G0 (nonstimulated) and G1 [phytohemagglutinin (PHA) stimulated] human lymphocytes by confocal microscopy. In addition to the nuclear center, the centrosome was used as a second point of reference in the three-dimensional (3D) analysis. Pericentromeric DNA of chromosome 11 and the centrosome were labeled using a combination of fluorescent in situ hybridization (FISH) and immunofluorescence. To preserve the 3D morphology of the cells, these techniques were performed on whole cells in suspension. Three-dimensional images of the cells were analyzed with a recently developed 3D software program (Interactive Measurement of Axes and Positioning in 3 Dimensions). The distribution of the chromosome 11 centromeres appeared to be random during the G0 stage but clearly non-random during the G1 stage, when the nuclear center was used as a reference point. Further statistical analysis of the G1 cells revealed that the centromeres were randomly distributed in a shell underlying the nuclear membrane. A topographical relationship between the centrosome and the centromeres appeared to be absent during the G0 and G1 stages of the cell cycle.

MeSH Terms
Cell Nucleus/metabolism Cell Separation Cells, Cultured Centromere Chromosomes, Human, Pair 11 DNA/metabolism Flow Cytometry G1 Phase Humans Lymphocytes/cytology,metabolism Resting Phase, Cell Cycle
Chemicals
DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hulspas R
Institute for Applied Radiobiology and Immunology TNO, Department of Molecular Pathology, Rijswijk, The Netherlands.
Houtsmuller A B
Krijtenburg P J
Bauman J G
Nanninga N
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00352253
Published
1994-07-00
Pages
286-92
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00352253 View journal details
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