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PMID: 7984422 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

GAGA factor and TBF1 bind DNA elements that direct ubiquitous transcription of the Drosophila alpha 1-tubulin gene.

Nucleic acids research ·Vol. 22 ·No. 22 ·1994-11-11 ·Pages 4712-8

O'Donnell KH, Wensink PC

Abstract

Three DNA regions (TE1, TE2 and the intron) regulate the ubiquitous expression of the alpha 1-tubulin gene of Drosophila melanogaster. In this report, we identify two proteins that bind these DNA regions. One is the previously characterized GAGA transcription factor and the other is a newly identified 62 kDa polypeptide, TBF1 (TE1-binding factor 1). Purified GAGA factor binds three sites in TE2 and at least three in the intron. TBF1 was purified from embryos and binds to both TE1 and TE2. Together, the two proteins produce the same DNase I footprints in TE1 and TE2 as does a nuclear extract that transcribes the gene accurately. These footprints cover most of the TE1 and TE2 DNA. Moreover, one binding site for each protein coincides with a site that activates transcription in vitro. The characteristics of the GAGA factor and the genes it regulates suggest roles these two proteins are likely to play in regulating ubiquitous expression.

MeSH Terms
Animals Base Sequence Binding Sites Cells, Cultured DNA/metabolism DNA-Binding Proteins/isolation & purification,metabolism Drosophila Proteins Drosophila melanogaster/embryology,metabolism Fungal Proteins/isolation & purification,metabolism Gene Expression Regulation Genes, Insect/genetics Homeodomain Proteins Introns Molecular Sequence Data Regulatory Sequences, Nucleic Acid/genetics Saccharomyces cerevisiae Proteins Transcription Factors/metabolism Transcription, Genetic/physiology Tubulin/genetics
Chemicals
DNA-Binding Proteins Drosophila Proteins Fungal Proteins Homeodomain Proteins Saccharomyces cerevisiae Proteins TBF1 protein, S cerevisiae Transcription Factors Trl protein, Drosophila Tubulin DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
O'Donnell K H
Department of Biochemistry, Brandeis University, Waltham, MA 02254-9110.
Wensink P C
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.22.4712
Published
1994-11-11
Pages
4712-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC308522
Subset
IM
Grants
NIGMS NIH HHS · GM21626 · United States
NIGMS NIH HHS · GM31234 · United States
External Links
PubMed source DOI: 10.1093/nar/22.22.4712 PMC Full Text View journal details
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