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PMID: 7983702 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

RNA binding properties of poliovirus subviral particles.

Journal of virology ·Vol. 69 ·No. 1 ·1995-01-00 ·Pages 13-22

Nugent CI, Kirkegaard K

Abstract

The mechanism of encapsidation of the RNA genome of poliovirus and other picornaviruses is unknown. To test whether any of the putative assembly intermediates of poliovirus could interact directly with the poliovirus RNA genome, poliovirus RNA was attached to magnetic streptavidin beads and incubated with partially purified extracts containing 35S-labeled 14S pentamer and 75S empty-capsid subviral particles from infected cells. The amount of labeled protein bound to the beads was monitored, thus testing the RNA-binding activities of only the labeled viral proteins in the preparations. In this assay, nonspecific RNA-binding activity was displayed by the 14S pentameric particles and mature virons. 75S empty capsids displayed no propensity to associate with RNA. 14S pentamers were demonstrated to form rapidly sedimenting complexes and to undergo a conformational alteration upon RNA binding. These findings are consistent with a direct role for the 14S pentameric particles in RNA packaging during poliovirus morphogenesis.

MeSH Terms
Binding Sites Capsid/metabolism HeLa Cells Humans Mutation Poliovirus/genetics,metabolism RNA, Viral/metabolism Virion/metabolism
Chemicals
RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nugent C I
Department of Molecular, Cellular and Developmental Biology, Howard Hughes Medical Institute, University of Colorado, Boulder 80309.
Kirkegaard K
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.69.1.13-22.1995
Published
1995-01-00
Pages
13-22
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC188543
Subset
IM
Grants
NIAID NIH HHS · AI-25166 · United States
External Links
PubMed source DOI: 10.1128/JVI.69.1.13-22.1995 PMC Full Text View journal details
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