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PMID: 7968268 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Stimulation of phosphatidylglycerolphosphate phosphatase activity by unsaturated fatty acids in rat heart.

Lipids ·Vol. 29 ·No. 7 ·1994-07-00 ·Pages 475-80

Cao SG, Hatch GM

Abstract

Phosphatidylglycerolphosphate (PGP) synthase and PGP phosphatase catalyze the sequential synthesis of phosphatidylglycerol from cytidine-5'-diphosphate 1,2-diacyl-sn-glycerol (CDP-DG) and glycerol-3-phosphate. PGP synthase and PGP phosphatase activities were characterized in rat heart mitochondrial fractions, and the effect of fatty acids on the activity of these enzymes was determined. PGP synthase was observed to be a heat labile enzyme that exhibited apparent Km values for CDP-PG and glycerol-3-phosphate of 46 and 20 microM, respectively. The addition of exogenous oleic acid to the assay mixture did not affect PGP synthase activity. PGP phosphatase was observed to be a heat labile enzyme, and addition of oleic acid to the assay mixture caused a concentration-dependent stimulation of PGP phosphatase activity. Maximum stimulation (1.9-fold) of enzyme activity was observed in the presence of 0.5 mM oleic acid, but the stimulation was slightly attenuated by the presence of albumin in the assay. The presence of oleic acid in the assay mixture caused the inactivation of PGP phosphatase activity to be retarded at 55 degrees C. Stimulation of PGP phosphatase activity was also observed with arachidonic acid, whereas taurocholic, stearic and palmitic acids did not significantly affect PGP phosphatase activity. The activity of mitochondrial phosphatidic acid phosphohydrolase was not affected by inclusion of oleic acid in the incubation mixture. We postulate that unsaturated fatty acids stimulate PGP phosphatase activity in rat heart.

MeSH Terms
Albumins/pharmacology Animals Fatty Acids, Unsaturated/pharmacology Hot Temperature Kinetics Male Mitochondria, Heart/enzymology Phosphatidylglycerols/metabolism Phosphoric Monoester Hydrolases/antagonists & inhibitors,metabolism Rats Rats, Sprague-Dawley Transferases (Other Substituted Phosphate Groups)/antagonists & inhibitors,metabolism
Chemicals
Albumins Fatty Acids, Unsaturated Phosphatidylglycerols phosphatidylglycerophosphate Transferases (Other Substituted Phosphate Groups) CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase Phosphoric Monoester Hydrolases phosphatidylglycerophosphatase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cao S G
Department of Internal Medicine, University of Manitoba, Winnipeg, Canada.
Hatch G M
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Article Info
Journal
Lipids
Abbr.
Lipids
ISSN
0024-4201
DOI
10.1007/BF02578244
Published
1994-07-00
Pages
475-80
Language
English
Region
United States
NLM ID
0060450
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02578244 View journal details
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