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PMID: 7966630 Published · ppublish English Journal Article

Effect of a single amino acid substitution in the V3 domain of the human immunodeficiency virus type 1: generation of revertant viruses to overcome defects in infectivity in specific cell types.

Journal of virology ·Vol. 68 ·No. 12 ·1994-12-00 ·Pages 8380-5

Morris JF, Sternberg EJ, Gutshall L, Petteway SR, Ivanoff LA

Abstract

Proviral clones of human immunodeficiency virus type 1 which contained single amino acid changes in the envelope V3 region were constructed. PCR amplification of Sup-T1 T cells transfected with one such mutant, G312T, revealed low levels of virus that resulted in the generation of a revertant virus, in which an alanine replaced the threonine residue at amino acid 312. The revertant virus (rA312) was fully infectious in Sup-T1 cells but lacked the ability to infect AA5 cells. The presence of a second mutation in a subsequent revertant virus (rR306), in which arginine was substituted for serine at amino acid 306 within the V3 loop, restored the ability of the mutated virus to infect AA5 cells. Our data highlight the importance of the V3 loop in defining virus tropism for specific cell types in culture and further suggest that a degree of interplay exists among V3 loop residues that helps maintain or control its biological function of the virus.

MeSH Terms
Animals Base Sequence Cell Line Cell-Free System Chlorocebus aethiops DNA Primers Gene Products, env/biosynthesis,metabolism HIV-1/pathogenicity,physiology Humans Molecular Sequence Data Mutagenesis, Site-Directed Point Mutation Polymerase Chain Reaction Proviruses/pathogenicity,physiology Restriction Mapping Transfection Virulence Virus Replication
Chemicals
DNA Primers Gene Products, env
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Morris J F
Department of Molecular Virology and Host Defense, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania 19406-0939.
Sternberg E J
Gutshall L
Petteway S R
Ivanoff L A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.12.8380-8385.1994
Published
1994-12-00
Pages
8380-5
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC237307
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.68.12.8380-8385.1994 PMC Full Text View journal details
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