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PMID: 7961477 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Molecular cloning and characterization of the aklavinone 11-hydroxylase gene of Streptomyces peucetius subsp. caesius ATCC 27952.

Journal of bacteriology ·Vol. 176 ·No. 22 ·1994-11-00 ·Pages 7096-101

Hong YS, Hwang CK, Hong SK, Kim YH, Lee JJ

Abstract

The gene encoding aklavinone 11-hydroxylase of Streptomyces peucetius subsp. caesius ATCC 27952 was cloned and sequenced. The deduced amino acid sequence of the gene contains at least two common motifs of well-conserved amino acid sequences of several flavin-type bacterial hydroxylases. The hydroxylase gene is apparently transcribed from a single transcriptional start point. The phenotype of a dnrF mutant generated by gene disruption supports the idea that the dnrF gene encodes aklavinone 11-hydroxylase.

Related Genes
dnrf
MeSH Terms
Amino Acid Sequence Aryl Hydrocarbon Hydroxylases/genetics Base Sequence Cloning, Molecular Flavin-Adenine Dinucleotide Genes, Fungal/genetics Molecular Sequence Data NAD Naphthacenes/metabolism Sequence Analysis, DNA Streptomyces/enzymology,genetics Transcription, Genetic
Chemicals
Naphthacenes NAD Flavin-Adenine Dinucleotide aklavinone aklavinone 11-hydroxylase Aryl Hydrocarbon Hydroxylases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hong Y S
Genetic Engineering Research Institute, Korea Institute of Science and Technology, Taejon.
Hwang C K
Hong S K
Kim Y H
Lee J J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.22.7096-7101.1994
Published
1994-11-00
Pages
7096-101
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC197087
Subset
IM
Databases
GENBANK
U09844
External Links
PubMed source DOI: 10.1128/jb.176.22.7096-7101.1994 PMC Full Text View journal details
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