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PMID: 7961454 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of distinct domains for signaling and receptor interaction of the sensory rhodopsin I transducer, HtrI.

Journal of bacteriology ·Vol. 176 ·No. 22 ·1994-11-00 ·Pages 6931-5

Yao VJ, Spudich EN, Spudich JL

Abstract

The phototaxis-deficient mutant of Halobacterium salinarium, Pho81, lacks both sensory rhodopsin I (SR-I) and its putative transducer protein HtrI, according to immunoblotting and spectroscopic criteria. From restriction analysis and selected DNA sequencing, we have determined that the SR-I- HtrI- phenotype results from an insertion of a 520-bp transposable element, ISH2, into the coding region of the SR-I apoprotein gene sopI and deletion of 11 kbp upstream of ISH2 including the first 164 bp of sopI and the entire htrI gene. SR-I and HtrI expression as well as full phototaxis sensitivity are restored by transformation with a halobacterial plasmid carrying the htrI-sopI gene pair and their upstream promoter region. An internal deletion of a portion of htrI encoding the putative methylation and signaling domains of HtrI (253 residues) prevents the restoration of phototaxis, providing further evidence for the role of HtrI as a transducer for SR-I. Analysis of flash-induced photochemical reactions of SR-I over a range of pH shows that the partially deleted HtrI maintains SR-I interactions sites responsible for modulation of the SR-I photocycle.

Related Genes
htrI sopI
MeSH Terms
Antibodies, Bacterial Archaeal Proteins Bacterial Proteins/biosynthesis,genetics,immunology Bacteriorhodopsins/genetics Base Sequence Cell Movement/genetics DNA Mutational Analysis Escherichia coli/genetics Halobacterium/genetics,radiation effects Halorhodopsins Light Membrane Proteins/biosynthesis,genetics,immunology Molecular Sequence Data Recombinant Proteins/biosynthesis Sensory Rhodopsins Signal Transduction/genetics Structure-Activity Relationship Transformation, Genetic
Chemicals
Antibodies, Bacterial Archaeal Proteins Bacterial Proteins Halorhodopsins Membrane Proteins Recombinant Proteins SRI protein, Halobacterium Sensory Rhodopsins htrI protein, Halobacterium salinarium Bacteriorhodopsins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yao V J
Department of Microbiology and Molecular Genetics, University of Texas Medical School Health Science Center, Houston 77030.
Spudich E N
Spudich J L
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22 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.22.6931-6935.1994
Published
1994-11-00
Pages
6931-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC197063
Subset
IM
Grants
NIGMS NIH HHS · 5T32GM07128 · United States
NIGMS NIH HHS · R01GM27750 · United States
External Links
PubMed source DOI: 10.1128/jb.176.22.6931-6935.1994 PMC Full Text View journal details
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