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PMID: 7945289 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

cDNA cloning and expression of bovine endothelin converting enzyme.

Biochemical and biophysical research communications ·Vol. 203 ·No. 3 ·1994-09-30 ·Pages 1417-22

Ikura T, Sawamura T, Shiraki T, Hosokawa H, Kido T, Hoshikawa H, Shimada K, Tanzawa K, Kobayashi S, Miwa S

Abstract

We have cloned cDNA encoding bovine endothelin converting enzyme (ECE). The predicted amino acid sequence of bovine ECE consisted of 758 amino acid residues. Northern blot analysis revealed that ECE mRNA was abundantly expressed in lung. Co-expression of the cloned cDNA of bovine ECE with human preproET-1 cDNA in CHO-K1 cells resulted in the production of mature ET-1 and this production was inhibited by phosphoramidon.

MeSH Terms
Amino Acid Sequence Animals Aspartic Acid Endopeptidases/antagonists & inhibitors,biosynthesis,metabolism Base Sequence Blotting, Northern CHO Cells Cattle Cloning, Molecular Cricetinae DNA, Complementary/metabolism Endothelin-1 Endothelin-Converting Enzymes Endothelins/biosynthesis,metabolism Gene Expression Glycopeptides/pharmacology Humans Lung/enzymology Metalloendopeptidases Molecular Sequence Data Protein Precursors/biosynthesis,metabolism RNA, Messenger/biosynthesis Recombinant Proteins/antagonists & inhibitors,biosynthesis Transfection
Chemicals
DNA, Complementary Endothelin-1 Endothelins Glycopeptides Protein Precursors RNA, Messenger Recombinant Proteins Aspartic Acid Endopeptidases Metalloendopeptidases Endothelin-Converting Enzymes phosphoramidon
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Ikura T
Department of Pharmacology, Faculty of Medicine, Kyoto University, Japan.
Sawamura T
Shiraki T
Hosokawa H
Kido T
Hoshikawa H
Shimada K
Tanzawa K
Kobayashi S
Miwa S
Article Info
Journal
Biochemical and biophysical research communications
Abbr.
Biochem Biophys Res Commun
ISSN
0006-291X
DOI
10.1006/bbrc.1994.2343
Published
1994-09-30
Pages
1417-22
Language
English
Region
United States
NLM ID
0372516
Subset
IM
Databases
GENBANK
S73774
External Links
PubMed source DOI: 10.1006/bbrc.1994.2343 View journal details
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