| GeneLibs

题目:: Paracrine interactions among parathyroid cells: effect of cell density on cell secretion.
作者:: Sun(F),Maercklein(P),Fitzpatrick(L A)
状态:: 发布时间1994-11-01 , 更新时间 2007-11-14
期刊:: J Bone Miner Res
摘要:: Cell-cell interactions are important in the regulation of endocrine cell secretion. To investigate the possibility that cell communication may alter the regulation of parathyroid cell secretion, we utilized the reverse hemolytic plaque assay (RHPA) to measure parathyroid hormone (PTH) release from individual cells. Bovine parathyroid cells were dispersed and plated with protein A-conjugated erythrocytes at cell densities ranging from 0.9 to 36 x 10(2) cells/cm2 in 0.2 mM calcium. Cell populations were greater than 98% homogenous as determined by immunocytochemistry and in situ hybridization for PTH mRNA. Plaques were developed and data analyzed for the amount of PTH per cell released (plaque area in microns 2 x 10(4)) and the determination of cell recruitment (% plaques formed). A positive correlation existed between parathyroid cell density and the amount of PTH released. As the distance between cells increased, the plaque area (amount of PTH released per cell) decreased (ranging from 1.0 x 10(4) microns 2 at 0.9 x 10(2) cells/cm2 versus 1.6 x 10(4) microns 2 at 36 x 10(2) cells/cm2). The percentage of cells releasing PTH (recruitment) also decreased (16% at 0.9 x 10(2) cells/cm2 versus 47% at 36 x 10(2) cells/cm2). These data suggest that parathyroid cells in close proximity are stimulated to secrete more hormone than those at lesser densities. In addition, parathyroid cells are recruited to secrete PTH when plated at high density. Factor(s) released by the parathyroid cell may increase cell responsiveness and stimulate secretion in a paracrine fashion.
语言:: eng
DOI:: 10.1002/jbmr.5650090703

文献库文献相关信息

DataTable pData

联系方式

微信公众号

文献库 文献相关信息

DataTable pData

联系方式

微信公众号

文献库文献相关信息