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PMID: 7937909 Published · ppublish English Comparative Study Journal Article

Iron acquisition from heme and hemoglobin by a Serratia marcescens extracellular protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 21 ·1994-10-11 ·Pages 9876-80

Létoffé S, Ghigo JM, Wandersman C

Abstract

Several pathogenic bacteria are able to use heme and hemoproteins as iron sources independent of siderophore production by mechanisms involving outer membrane heme-binding proteins and heme transport systems. Here we show that Serratia marcescens has such a property and we identify an extracellular heme-binding protein, HasA (for heme acquisition system), allowing the release of heme from hemoglobin. This protein is secreted by S. marcescens under conditions of iron depletion and is essential for heme acquisition.

Related Genes
hasA kan prtD prtD<up>SM</up> psmX sptE<up>SM</up>
MeSH Terms
Amino Acid Sequence Base Sequence Carrier Proteins/genetics,isolation & purification,metabolism Chromatography, Affinity DNA, Bacterial/chemistry,metabolism Electrophoresis, Polyacrylamide Gel Escherichia coli/growth & development,metabolism Genes, Bacterial Heme/metabolism Hemoglobins/metabolism Iron/metabolism Molecular Sequence Data Protein Binding Serratia marcescens/genetics,growth & development,metabolism
Chemicals
Carrier Proteins DNA, Bacterial Hemoglobins Heme Iron
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Létoffé S
Unité de Physiologie Cellulaire, Centre National de la Recherche Scientifique, Unité de Recherche Associée 1300, Institut Pasteur, Paris, France.
Ghigo J M
Wandersman C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.21.9876
Published
1994-10-11
Pages
9876-80
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44920
Subset
IM
Databases
GENBANK
X81195
External Links
PubMed source DOI: 10.1073/pnas.91.21.9876 PMC Full Text View journal details
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