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PMID: 7937868 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transfection of an Fc gamma receptor cDNA induces T cells to become phagocytic.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 21 ·1994-10-11 ·Pages 10232-6

Hunter S, Kamoun M, Schreiber AD

Abstract

The human receptor Fc gamma RIIA for the Fc portion of IgG (Fc gamma) was expressed in a human T-cell line and conferred on these cells the ability to perform IgG antibody-stimulated phagocytosis. Crosslinking Fc gamma RIIA with anti-Fc gamma RII monoclonal antibody also induced tyrosine phosphorylation of multiple proteins including Fc gamma RIIA, ZAP-70, p72SYK, and phospholipase C gamma 1 subunit and an increase in intracellular Ca2+ concentration. The T cell receptor-associated zeta-chain was not tyrosine-phosphorylated after crosslinking of Fc gamma RIIA, suggesting that the Fc gamma RIIA-mediated signals were independent of CD3. Fc gamma RIIA-mediated signal transduction was defective in a transfected mutant T-cell line exhibiting reduced expression of the tyrosine kinases LCK and FYN. These studies indicate that certain T cells can assume phagocytic properties after transfection of cDNA encoding an Fc gamma receptor with the capability of inducing a phagocytic signal.

MeSH Terms
Antibodies, Monoclonal/pharmacology CD3 Complex/isolation & purification,physiology Calcium/metabolism Cell Line Humans Interleukin-2/analysis,biosynthesis Mutagenesis, Site-Directed Phagocytosis/immunology Phosphoproteins/isolation & purification,metabolism Phosphorylation Receptors, IgG/biosynthesis,isolation & purification,physiology Signal Transduction T-Lymphocytes/immunology Transfection Tumor Cells, Cultured Type C Phospholipases/metabolism
Chemicals
Antibodies, Monoclonal CD3 Complex Interleukin-2 Phosphoproteins Receptors, IgG Type C Phospholipases Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hunter S
Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104.
Kamoun M
Schreiber A D
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.21.10232
Published
1994-10-11
Pages
10232-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44992
Subset
IM
Grants
NIAID NIH HHS · AI-20846 · United States
NIAID NIH HHS · AI/HL-22193 · United States
External Links
PubMed source DOI: 10.1073/pnas.91.21.10232 PMC Full Text View journal details
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