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PMID: 7937796 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and characterization of a Neurospora crassa gene required for (1,3) beta-glucan synthase activity and cell wall formation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 20 ·1994-09-27 ·Pages 9500-4

Enderlin CS, Selitrennikoff CP

Abstract

The glucan synthase 1 gene (gs-1) is required for (1,3) beta-glucan synthase activity [E.C. 2.4.1.34; UDP glucose:1,3-beta-D-glucan 3-beta-D-glucosyltransferase] and for cell wall formation. The gs-1 gene was cloned by functional complementation of the cell-wall-less defect of the (1,3) beta-glucan synthase-deficient mutant, TM1, by using a genomic Neurospora crassa cosmid library. A 2568-nucleotide gs-1 cDNA sequence revealed a 532-amino acid open reading frame encoding a polypeptide of 59 kDa. The predicted gs-1 gene product has no obvious signal peptide cleavage sites or transmembrane domains. A gs-1 null mutant is defective for cell wall formation and (1,3) beta-glucan synthase activity. The predicted GS-1 protein is weakly homologous to a putative Saccharomyces cerevisiae transcriptional regulatory protein.

Related Genes
gs-1
MeSH Terms
Amino Acid Sequence Base Sequence Cell Wall/genetics Cloning, Molecular DNA, Fungal/chemistry Genes, Fungal Glucosyltransferases/biosynthesis Membrane Proteins Molecular Sequence Data Neurospora crassa/enzymology,genetics Restriction Mapping Saccharomyces cerevisiae/genetics Schizosaccharomyces pombe Proteins Sequence Homology, Amino Acid
Chemicals
DNA, Fungal Membrane Proteins Schizosaccharomyces pombe Proteins Glucosyltransferases 1,3-beta-glucan synthase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Enderlin C S
Department of Cellular and Structural Biology, University of Colorado Health Sciences Center, Denver 80262.
Selitrennikoff C P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.20.9500
Published
1994-09-27
Pages
9500-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44840
Subset
IM
Databases
GENBANK
U09275
External Links
PubMed source DOI: 10.1073/pnas.91.20.9500 PMC Full Text View journal details
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