Ultrastructural localization of prolactin, growth hormone and luteinizing hormone by immunocytochemical techniques in the bovine pituitary.

Cell and tissue research ·Vol. 174 ·No. 2 ·1976-10-29 ·Pages 245-60

Dacheux F, Dubois MP

Abstract

The technique of ultrastructural immunocytochemistry involving the unlabeled antibody and the soluble peroxidase-antiperoxidase complex was used to identify and describe the prolactin (P) cells, somatotropic (STH) cells and luteinizing hormone (LH) cells in the bovine anterior pituitary gland. This method was used to localize the three hormones at the electron microscopic level. Staining of varying intensity was found on the secretory granules and on the small granules and vesicles within the Golgi complex. No stain was found in nuclei, on mitochondria or in the endoplasmic reticulum.

MeSH Terms

Animals Cattle Cytoplasmic Granules/analysis Female Golgi Apparatus/analysis Growth Hormone/analysis Immunoenzyme Techniques Luteinizing Hormone/analysis Male Microscopy, Electron Pituitary Gland/ultrastructure Pituitary Gland, Anterior/analysis,ultrastructure Prolactin/analysis

Chemicals

Prolactin Luteinizing Hormone Growth Hormone

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Dacheux F

Dubois M P

References (21)

21 references, click to expand

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Article Info

Journal

Cell and tissue research

Abbr.

Cell Tissue Res

ISSN

0302-766X

DOI

10.1007/BF00222162

Published

1976-10-29

Pages

245-60

Language

English

Region

Germany

NLM ID

0417625

Subset

External Links

PubMed source DOI: 10.1007/BF00222162 View journal details

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Ultrastructural localization of prolactin, growth hormone and luteinizing hormone by immunocytochemical techniques in the bovine pituitary.

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