Measurement of receptor-stimulated guanosine 5'-O-(gamma-thio)triphosphate binding by G proteins.

Animals Binding, Competitive Cell Membrane/metabolism Centrifugation, Density Gradient/methods Erythrocyte Membrane/metabolism GTP-Binding Proteins/isolation & purification,metabolism Guanosine 5'-O-(3-Thiotriphosphate)/metabolism Guanosine Diphosphate/pharmacology Humans Indicators and Reagents Kinetics Macromolecular Substances Radioisotope Dilution Technique Receptors, Adrenergic, beta/metabolism Receptors, Cell Surface/agonists,metabolism Receptors, Muscarinic/metabolism Sodium Chloride/pharmacology Sulfur Radioisotopes Temperature Turkeys

Indicators and Reagents Macromolecular Substances Receptors, Adrenergic, beta Receptors, Cell Surface Receptors, Muscarinic Sulfur Radioisotopes Guanosine Diphosphate Guanosine 5'-O-(3-Thiotriphosphate) Sodium Chloride GTP-Binding Proteins