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PMID: 7928998 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Isolation and sequence analysis of polyketide synthase genes from the daunomycin-producing Streptomyces sp. strain C5.

Journal of bacteriology ·Vol. 176 ·No. 20 ·1994-10-00 ·Pages 6270-80

Ye J, Dickens ML, Plater R, Li Y, Lawrence J, Strohl WR

Abstract

A contiguous region of about 30 kbp of DNA putatively encoding reactions in daunomycin biosynthesis was isolated from Streptomyces sp. strain C5 DNA. The DNA sequence of an 8.1-kbp EcoRI fragment, which hybridized with actI polyketide synthase (PKS) and actIII polyketide reductase (PKR) gene probes, was determined, revealing seven complete open reading frames (ORFs), two in one cluster and five in a divergently transcribed cluster. The former two genes are likely to encode PKR and a bifunctional cyclase/dehydrase. The five latter genes encode: (i) a homolog of TcmH, an oxygenase of the tetracenomycin biosynthesis pathway; (ii) a PKS Orf1 homolog; (iii) a PKS Orf2 homolog (chain length factor); (iv) a product having moderate sequence identity with Escherichia coli beta-ketoacyl acyl carrier protein synthase III but lacking the conserved active site; and (v) a protein highly similar to several acyltransferases. The DNA within the 8.1-kbp EcoRI fragment restored daunomycin production to two dauA non-daunomycin-producing mutants of Streptomyces sp. strain C5 and restored wild-type antibiotic production to Streptomyces coelicolor B40 (act VII; nonfunctional cyclase/dehydrase), and to S. coelicolor B41 (actIII) and Streptomyces galilaeus ATCC 31671, strains defective in PKR activity.

MeSH Terms
Amino Acid Sequence Base Sequence Daunorubicin/biosynthesis Genes, Bacterial/genetics Genetic Complementation Test Genomic Library Molecular Sequence Data Multienzyme Complexes/genetics Multigene Family/genetics Oligonucleotide Probes Open Reading Frames Restriction Mapping Sequence Analysis, DNA Sequence Homology, Amino Acid Species Specificity Streptomyces/genetics
Chemicals
Multienzyme Complexes Oligonucleotide Probes Daunorubicin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ye J
Department of Microbiology, Ohio State University, Columbus 43210-1292.
Dickens M L
Plater R
Li Y
Lawrence J
Strohl W R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.20.6270-6280.1994
Published
1994-10-00
Pages
6270-80
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC196968
Subset
IM
Grants
PHS HHS · 43345 · United States
Databases
GENBANK
L34880
External Links
PubMed source DOI: 10.1128/jb.176.20.6270-6280.1994 PMC Full Text View journal details
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