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PMID: 7926829 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The Schizosaccharomyces pombe rad1 gene consists of three exons and the cDNA sequence is partially homologous to the Ustilago maydis REC1 cDNA.

Gene ·Vol. 148 ·No. 1 ·1994-10-11 ·Pages 155-9

Long KE, Sunnerhagen P, Subramani S

Abstract

We show that the rad1 gene of Schizosaccharomyces pombe is comprised of three exons and encodes a protein of 37 kDa. A cDNA clone containing these three exons complements the sensitivity of the rad1-1 mutant to ultraviolet and gamma-radiation and to hydroxyurea. The newly identified ORF of the rad1 gene was found to exhibit partial homology to the REC1 gene of Ustilago maydis. These two genes share putative functional similarities in their respective organisms.

Related Genes
REC1 rad1
MeSH Terms
Amino Acid Sequence Base Sequence DNA, Complementary/genetics DNA-Binding Proteins Endonucleases Exodeoxyribonuclease V Exodeoxyribonucleases/genetics Exons/genetics Fungal Proteins/chemistry,genetics Gamma Rays Genetic Complementation Test Molecular Sequence Data Phenotype Point Mutation/physiology Schizosaccharomyces/genetics,radiation effects Schizosaccharomyces pombe Proteins Sequence Alignment Sequence Analysis, DNA Sequence Homology, Nucleic Acid Ultraviolet Rays Ustilago/genetics
Chemicals
DNA, Complementary DNA-Binding Proteins Fungal Proteins Schizosaccharomyces pombe Proteins Endonucleases Exodeoxyribonucleases rad1 protein, S pombe Exodeoxyribonuclease V
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Long K E
Department of Biology, University of California at San Diego, La Jolla 92093-0322.
Sunnerhagen P
Subramani S
Article Info
Journal
Gene
Abbr.
Gene
ISSN
0378-1119
DOI
10.1016/0378-1119(94)90250-x
Published
1994-10-11
Pages
155-9
Language
English
Region
Netherlands
NLM ID
7706761
Subset
IM
Grants
NIGMS NIH HHS · GM31253 · United States
Databases
GENBANK
M38132, U01836, Z28385
External Links
PubMed source DOI: 10.1016/0378-1119(94)90250-x View journal details
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