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PMID: 7915835 Published · ppublish English Journal Article

Direct sequencing of PCR products in agarose gel slices.

Nucleic acids research ·Vol. 22 ·No. 16 ·1994-08-25 ·Pages 3425-6

Khorana S, Gagel RF, Cote GJ

Abstract

暂无摘要

MeSH Terms
Base Sequence DNA/chemistry Electrophoresis, Agar Gel Hot Temperature Humans Leukocytes/chemistry Multiple Endocrine Neoplasia/genetics Nucleic Acid Denaturation Polymerase Chain Reaction Sepharose Sequence Analysis, DNA/methods Templates, Genetic
Chemicals
DNA Sepharose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Khorana S
Section of Endocrinology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Gagel R F
Cote G J
References (8)
8 references, click to expand
  1. Optimal conditions for directly sequencing double-stranded PCR products with sequenase.
    Nucleic Acids Res. 1990 Jul 11;18(13):4028 PMID: 2374739
  2. Production of single-stranded DNA for sequencing: an alternative approach.
    Biotechniques. 1992 Mar;12(3):346-50 PMID: 1349213
  3. Avoiding strand reassociation in direct sequencing of double-stranded PCR products with thermolabile polymerases.
    PCR Methods Appl. 1992 Feb;1(3):193-4 PMID: 1282068
  4. Specific mutations of the RET proto-oncogene are related to disease phenotype in MEN 2A and FMTC.
    Nat Genet. 1994 Jan;6(1):70-4 PMID: 7907913
  5. Comparison of some procedures to recover the PCR-amplified products for a direct sequencing.
    Genet Anal Tech Appl. 1993 Feb;10(1):24-6 PMID: 8329221
  6. Mutations in the RET proto-oncogene are associated with MEN 2A and FMTC.
    Hum Mol Genet. 1993 Jul;2(7):851-6 PMID: 8103403
  7. Direct sequencing of double-stranded PCR products isolated from conventional agarose gels.
    Biotechniques. 1993 Sep;15(3):384-6 PMID: 8217145
  8. Sequencing of PCR-amplified DNA.
    PCR Methods Appl. 1992 May;1(4):222-8 PMID: 1477656
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.16.3425
Published
1994-08-25
Pages
3425-6
Language
English
Region
England
NLM ID
0411011
PMCID
PMC523740
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/22.16.3425 PMC Full Text View journal details
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