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PMID: 7915099 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Revised analysis of aadA2 gene of plasmid pSa.

Antimicrobial agents and chemotherapy ·Vol. 38 ·No. 5 ·1994-05-00 ·Pages 1172-5

Bito A, Susani M

Abstract

The nucleotide sequence of gene aadA2 of plasmid pSa, coding for aminoglycoside-3"-adenyltransferase, has been reexamined. We found differences with respect to the sequence previously determined by Tait et al. (R. C. Tait, H. Rempel, R. L. Rodriguez, and C. I. Kado, Gene 36:97-104, 1985). These deviations are located in the coding region and in the 3' noncoding region. By making deletions in the region for initiation of protein synthesis, we identified a GTG triplet as the most probable start codon for translation.

Related Genes

aadA2

MeSH Terms

Amino Acid Sequence Anti-Bacterial Agents/pharmacology Base Sequence Cloning, Molecular Codon Drug Resistance, Microbial Molecular Sequence Data Nucleotidyltransferases/genetics Plasmids/genetics Polymorphism, Restriction Fragment Length Spectinomycin/pharmacology Streptomycin/pharmacology

Chemicals

Anti-Bacterial Agents Codon Spectinomycin Nucleotidyltransferases streptomycin 3''-adenylyltransferase Streptomycin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Bito A

Institut für Genetik und Allgemeine Biologie, Universität Salzburg, Austria.

Susani M

References (25)

25 references, click to expand

Site-specific insertion of gene cassettes into integrons.
Mol Microbiol. 1993 Jul;9(1):41-52 PMID: 8412670
The integron In1 in plasmid R46 includes two copies of the oxa2 gene cassette.
Plasmid. 1992 Nov;28(3):225-34 PMID: 1334268
Formation of an RNA primer for initiation of replication of ColE1 DNA by ribonuclease H.
Proc Natl Acad Sci U S A. 1980 May;77(5):2450-4 PMID: 6156450
Characterization of translational initiation sites in E. coli.
Nucleic Acids Res. 1982 May 11;10(9):2971-96 PMID: 7048258
Broad-host-range cloning vectors derived from the W-plasmid Sa.
Gene. 1982 Oct;19(3):361-4 PMID: 6295888
Nucleotide sequence analysis of a gene encoding a streptomycin/spectinomycin adenylyltransferase.
Plasmid. 1985 Jan;13(1):17-30 PMID: 2986186
Nucleotide sequence of the transposon Tn7 gene encoding an aminoglycoside-modifying enzyme, 3"(9)-O-nucleotidyltransferase.
Nucleic Acids Res. 1985 Oct 11;13(19):7095-106 PMID: 2997737
The aminoglycoside-resistance operon of the plasmid pSa: nucleotide sequence of the streptomycin-spectinomycin resistance gene.
Gene. 1985;36(1-2):97-104 PMID: 2998941
Characterization of transferable plasmids from Shigella flexneri 2a that confer resistance to trimethoprim, streptomycin, and sulfonamides.
Plasmid. 1986 Mar;15(2):119-31 PMID: 3517903
Plasmid vector pBR322 and its special-purpose derivatives--a review.
Gene. 1986;50(1-3):3-40 PMID: 3034735
DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767-71 PMID: 3474623
Precise insertion of antibiotic resistance determinants into Tn21-like transposons: nucleotide sequence of the OXA-1 beta-lactamase gene.
Proc Natl Acad Sci U S A. 1987 Nov;84(21):7378-82 PMID: 2823258
On the evolution of Tn21-like multiresistance transposons: sequence analysis of the gene (aacC1) for gentamicin acetyltransferase-3-I(AAC(3)-I), another member of the Tn21-based expression cassette.
Mol Gen Genet. 1989 Jun;217(2-3):202-8 PMID: 2549372
A novel family of potentially mobile DNA elements encoding site-specific gene-integration functions: integrons.
Mol Microbiol. 1989 Dec;3(12):1669-83 PMID: 2560119
Genetic elements involved in Tn21 site-specific integration, a novel mechanism for the dissemination of antibiotic resistance genes.
EMBO J. 1990 Apr;9(4):1275-81 PMID: 2157593
The dhfrI trimethoprim resistance gene of Tn7 can be found at specific sites in other genetic surroundings.
Antimicrob Agents Chemother. 1990 Apr;34(4):642-50 PMID: 2188588
Sequencing and expression of aadA, bla, and tnpR from the multiresistance transposon Tn1331.
Plasmid. 1990 Nov;24(3):218-26 PMID: 1963948
Characterization of the nonenzymatic chloramphenicol resistance (cmlA) gene of the In4 integron of Tn1696: similarity of the product to transmembrane transport proteins.
J Bacteriol. 1991 Jul;173(14):4493-502 PMID: 1648560
Aminoglycoside-3''-adenyltransferase confers resistance to spectinomycin and streptomycin in Nicotiana tabacum.
Plant Mol Biol. 1990 Feb;14(2):197-205 PMID: 1966273
Sequence analysis of the inducible chloramphenicol resistance determinant in the Tn1696 integron suggests regulation by translational attenuation.
Plasmid. 1991 Jul;26(1):10-9 PMID: 1658833
Site-specific insertion of genes into integrons: role of the 59-base element and determination of the recombination cross-over point.
Mol Microbiol. 1991 Aug;5(8):1941-59 PMID: 1662753
Site-specific deletion and rearrangement of integron insert genes catalyzed by the integron DNA integrase.
J Bacteriol. 1992 Mar;174(5):1574-85 PMID: 1311297
Isolation and characterization of the aadA aminoglycoside-resistance gene from Salmonella choleraesuis.
Mol Microbiol. 1992 Sep;6(17):2453-60 PMID: 1406282
Gene cassettes from the insert region of integrons are excised as covalently closed circles.
Mol Microbiol. 1992 Oct;6(19):2875-85 PMID: 1331702
The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299

Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.38.5.1172

Published

1994-05-00

Pages

1172-5

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC188172

Subset

Databases

GENBANK

X68227

External Links

PubMed source DOI: 10.1128/AAC.38.5.1172 PMC Full Text View journal details

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Revised analysis of aadA2 gene of plasmid pSa.

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