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题目:: Giant cell tumor of bone: analysis of proliferative cells by double-labeling immunohistochemistry with anti-proliferating cell nuclear antigen antibody and culture procedure.
作者:: Abe(Y),Yonemura(K),Nishida(K),Takagi(K)
状态:: 发布时间1994-09-06 , 更新时间 2011-07-27
期刊:: Nihon Seikeigeka Gakkai Zasshi
摘要:: Giant cell tumor of bone (GCT) consists of multinucleated giant cells, fibroblast-like cells, and macrophage-like cells, but what constitutes the principal neoplastic cells and their histogenesis remains controversial. Proliferating cells in GCT have been immunophenotypically characterized with the cell cycle-related antigen proliferating cell nuclear antigen (PCNA). In seven cases, PCNA monoclonal antibody was applied by double-labeling immunohistochemical methods together with other cell-specific monoclonal antibodies. Many proliferating PCNA-positive cells were found in the vimentin-positive fibroblast-like cells, while almost all macrophage-like cells were negative for PCNA. The study of cultured cells showed that the fibroblast-like spindle-shaped cells were a neoplastic element. In addition, osteoclast-like cells were detected in co-culture systems of cells cultured from GCT with spleen cells. These studies support the concept that GCT is seen as a neoplasm of fibroblast-like cells with a strong reactive infiltration of cells from the mononuclear phagocyte system.
语言:: eng
DOI:

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