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PMID: 7904723 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning and characterization of centromeric DNA from Neurospora crassa.

Molecular and cellular biology ·Vol. 14 ·No. 2 ·1994-02-00 ·Pages 1510-9

Centola M, Carbon J

Abstract

The centromere locus from linkage group VII of Neurospora crassa has been cloned, characterized, and physically mapped. The centromeric DNA is contained within a 450-kb region that is recombination deficient, A+T-rich, and contains repetitive sequences. Repetitive sequences from within this region hybridize to a family of repeats located at or near centromeres in all seven linkage groups of N. crassa. Genomic Southern blots and sequence analysis of these repeats revealed a unique centromere structure containing a divergent family of centromere-specific repeats. The predominantly transitional differences between copies of the centromere-specific sequence repeats and their high A+T content suggest that their divergence was mediated by repeat-induced point (RIP) mutations.

MeSH Terms
Base Composition Base Sequence Blotting, Southern Centromere/chemistry,physiology Chromosome Walking Chromosomes, Artificial, Yeast Chromosomes, Fungal Cloning, Molecular/methods DNA, Fungal/chemistry,genetics Deoxyribonucleases, Type II Site-Specific Escherichia coli Genetic Linkage Molecular Sequence Data Neurospora crassa/genetics Polymorphism, Restriction Fragment Length Repetitive Sequences, Nucleic Acid Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
DNA, Fungal Deoxyribonucleases, Type II Site-Specific GGTACC-specific type II deoxyribonucleases endodeoxyribonuclease PacI
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Centola M
Department of Biological Sciences, University of California, Santa Barbara 93106.
Carbon J
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32 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.2.1510-1519.1994
Published
1994-02-00
Pages
1510-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358506
Subset
IM
Grants
NCI NIH HHS · CA-11034 · United States
Databases
GENBANK
L23168
External Links
PubMed source DOI: 10.1128/mcb.14.2.1510-1519.1994 PMC Full Text View journal details
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