Convenient method for detecting 14CO2 in multiple samples: application to rapid screening for mutants.

Journal of bacteriology ·Vol. 128 ·No. 1 ·1976-10-00 ·Pages 485-6

Tabor H, Tabor CW, Hafner EW

Abstract

A procedure is presented for the rapid screening of bacterial colonies to detect mutants unable to produce 14CO2 from a labeled precursor. The method is especially useful for mass screening for mutants that cannot be easily detected by their phenotypic characteristics.

MeSH Terms

Adenosylmethionine Decarboxylase/metabolism Bacteriological Techniques Carbon Dioxide/metabolism Escherichia coli/metabolism Mutation Temperature

Chemicals

Carbon Dioxide Adenosylmethionine Decarboxylase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Tabor H

Tabor C W

Hafner E W

References (5)

5 references, click to expand

Isolation, characterization, and mapping of Escherichia coli mutants blocked in the synthesis of ornithine decarboxylase.
J Bacteriol. 1975 Nov;124(2):791-9 PMID: 1102531
Mass screening for mutants with altered DNases by microassay techniques.
Methods Enzymol. 1974;29:180-93 PMID: 4604728
Multiple pathways of putrescine biosynthesis in Escherichia coli.
J Biol Chem. 1966 Jul 10;241(13):3129-35 PMID: 5330264
Urea production and putrescine biosynthesis by Escherichia coli.
J Bacteriol. 1967 Nov;94(5):1516-9 PMID: 4862195
Purification of adenosylmethionine decarboxylase from Escherichia coli W: evidence for covalently bound pyruvate.
J Biol Chem. 1970 Apr 25;245(8):2132-9 PMID: 4909563

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.128.1.485-486.1976

Published

1976-10-00

Pages

485-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC232878

Subset

External Links

PubMed source DOI: 10.1128/jb.128.1.485-486.1976 PMC Full Text View journal details

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Convenient method for detecting 14CO2 in multiple samples: application to rapid screening for mutants.

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