Characterization and occurrence of two repeated palindromic DNA elements of Brucella spp.: Bru-RS1 and Bru-RS2.

Molecular microbiology ·Vol. 14 ·No. 4 ·1994-11-00 ·Pages 681-9

Halling SM, Bricker BJ

Abstract

Two repeated DNA elements of 103 bp and 105 bp were discovered in brucellae and designated Bru-RS1 and Bru-RS2, respectively. The two elements are palindromic, are 65% similar in sequence, form two families of elements that are slightly divergent in sequence, appear to be intergenic, and are found, collectively, in more than 35 copies in brucellae. These elements are bounded by perfect or nearly perfect inverted repeats. A third copy of the terminal repeat is found within the elements and is the terminus for several truncated copies of the Bru-RS1 family. Hybridization patterns for the elements among brucellae were unique. The elements are dispersed, highly conserved among brucellae, and hot-spots for insertion by IS711.

MeSH Terms

Base Sequence Brucella/genetics Brucella abortus/chemistry DNA Primers/genetics DNA Transposable Elements DNA, Bacterial/chemistry,genetics Gene Amplification Genes, Bacterial Molecular Sequence Data Nucleic Acid Conformation Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid

Chemicals

DNA Primers DNA Transposable Elements DNA, Bacterial

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Halling S M

United States Department of Agriculture, National Animal Disease Center, Ames, Iowa 50010.

Bricker B J

Article Info

Journal

Molecular microbiology

Abbr.

Mol Microbiol

ISSN

0950-382X

DOI

10.1111/j.1365-2958.1994.tb01306.x

Published

1994-11-00

Pages

681-9

Language

English

Region

England

NLM ID

8712028

Subset

Databases

GENBANK

L24471, L24472, L24473, L24474, L24475, L24476, L24477, L24478, L24479, L24480, L24481, L24482, L24483, L24484, L24485, L24486

External Links

PubMed source DOI: 10.1111/j.1365-2958.1994.tb01306.x View journal details

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Characterization and occurrence of two repeated palindromic DNA elements of Brucella spp.: Bru-RS1 and Bru-RS2.

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