The use of K(+)-free buffers eliminates a common cause of premature chain termination in PCR and PCR sequencing.

Nucleic acids research ·Vol. 23 ·No. 3 ·1995-02-11 ·Pages 539

Woodford K, Weitzmann MN, Usdin K

Abstract

暂无摘要

MeSH Terms

Base Sequence Buffers Molecular Sequence Data Polymerase Chain Reaction/methods Potassium Chloride RNA Polymerase II/genetics Sequence Analysis, DNA/methods

Chemicals

Buffers Potassium Chloride RNA Polymerase II

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Woodford K

Section on Genomic Structure and Function, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0830.

Weitzmann M N

Usdin K

References (4)

4 references, click to expand

A sodium-potassium switch in the formation of four-stranded G4-DNA.
Nature. 1990 Mar 29;344(6265):410-4 PMID: 2320109
A novel K(+)-dependent DNA synthesis arrest site in a commonly occurring sequence motif in eukaryotes.
J Biol Chem. 1994 Oct 28;269(43):27029-35 PMID: 7929444
An effective method for eliminating "artifact banding" when sequencing double-stranded DNA templates.
Biotechniques. 1990 Jul;9(1):46-8 PMID: 1697472
The amino acid sequence of the human RNA polymerase II 33-kDa subunit hRPB 33 is highly conserved among eukaryotes.
J Biol Chem. 1990 May 25;265(15):8400-3 PMID: 2187864

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/23.3.539

Published

1995-02-11

Pages

539

Language

English

Region

England

NLM ID

0411011

PMCID

PMC306710

Subset

External Links

PubMed source DOI: 10.1093/nar/23.3.539 PMC Full Text View journal details

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The use of K(+)-free buffers eliminates a common cause of premature chain termination in PCR and PCR sequencing.

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